大量流行病学研究表明，运动与不同类型癌症的风险降低存在关系。体外研究已经确定运动适应的人类血清对肺癌、乳腺癌、前列腺癌和结肠癌细胞系具有直接影响。这项系统综述和荟萃分析（SRM）的目的是估计运动适应的人类血清对癌细胞培养活性的影响程度。该设计遵循PRISMA准则和TREND声明，以评估每个研究中信息的质量（QoI）。SRM包括了9个体外研究，涉及9种癌细胞系和来自不同国家的244名个体的血清，包括健康久坐的个体、前列腺癌风险个体和癌症患者，年龄范围从18岁到73岁。通过多种试验使用前运动人类血清进行比较，分析运动适应的人类血清对癌细胞培养活性的影响。总体而言，与对照组相比，暴露于不同强度的运动适应的人类血清的癌细胞系培养物表现出较低的存活率，整体效应大小为-1.126（95％CI；-1.300至-0.952；p＜0.001） 。当分析仅包括高强度运动适应的人类血清时，效应更加显著（ES-1.350；-1.522至-1.179（95％CI）; p＜0.001）。这些结果符合一个假设，即运动引起的人类血清变化可能在癌症预防和治疗中发挥作用，并且这些效应取决于运动强度。 版权所有©2021国际运动与免疫学学会。保留所有权利。

Numerous epidemiological studies have shown the existence of a relationship between exercise and reduced risk of different types of cancer. In vitro studies have identified a direct effect of exercise-conditioned human serum on cancer cell lines of the lung, breast, prostate, and colon. The aim of this systematic review with meta-analysis (SRM) was to estimate the magnitude of the effect that exercise-conditioned human serum produced on the viability of cancer cell cultures. The design followed the PRISMA guidelines and the TREND statement to assess the quality of information (QoI) in each study. Nine in vitro studies were included in the SRM, involving a total of nine cancer cell lines and serum from 244 individuals from different countries, including namely healthy sedentary individuals, at risk of prostate cancer individuals and cancer patients, with ages ranging from 18 to 73 years. The impact of exerciseconditioned human serum on the viability of cancer cell cultures was analysed by a variety of assays, using pre-exercise human serum for comparison purposes. Globally, cultures of cancer cell lines exposed to human serum conditioned by exercise of various intensities exhibited a reduced viability, when compared with control cultures, with an overall effect size of -1.126 (95% CI; -1.300 to -0.952; p < 0.001). When the analysis only included human serum conditioned by high intensity exercise, the effect became more pronounced (ES -1.350; -1.522 to -1.179 (95% CI); p < 0.001). These results are in line with the hypothesis that changes in human serum induced by exercise might play a role in the beneficial effects of physical activity in cancer prevention and management and that these effects depend on exercise intensity.Copyright © 2021 International Society of Exercise and Immunology. All rights reserved.