器官芽成像: 观察发育和功能。
Organoid Imaging: Seeing Development and Function.
发表日期：2022 Oct 06
作者：
Rashmiparvathi Keshara, Yung Hae Kim, Anne Grapin-Botton
来源：
Annual Review of Cell and Developmental Biology
摘要：
Organoids是在体外由干细胞或祖细胞生产的器官的微型化简版。它们被用作模型系统，由多种细胞类型形成与器官相关的结构并执行器官的功能。它们是研究发育、平衡、再生和疾病的有用工具。器官小体的成像已成为可视化和理解其自组织、对称破缺、生长、分化和功能的关键方法。在本文中，我们讨论成像方法、如何分析这些图像以及器官小体研究中面临的挑战。
Organoids are miniaturized and simplified versions of an organ produced in vitro from stem or progenitor cells. They are used as a model system consisting of multiple cell types forming an architecture relevant to the organ and carrying out the function of the organ. They are a useful tool to study development, homeostasis, regeneration, and disease. The imaging of organoids has become a pivotal method to visualize and understand their self-organization, symmetry breaking, growth, differentiation, and function. In this review, we discuss imaging methods, how to analyze these images, and challenges in organoid research.