Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer. 聚乙烯吡咯烷酮稳定的银纳米颗粒具有潜在的对前列腺癌的抗癌活性。
Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer.
发表日期：2022
作者：
Ahmed A H Abdellatif, Ahmed Abdelfattah, Abdellatif Bouazzaoui, Shaaban K Osman, Issa Saad Al-Moraya, Abdulaziz M Saleh Showail, Mansour Alsharidah, Ashraf Aboelela, Osamah Al Rugaie, Tarek M Faris, Hesham M Tawfeek
来源：
BIOINORGANIC CHEMISTRY AND APPLICATIONS
摘要：
肿瘤坏死因子（TNF-α）和炎性细胞因子（IL-6）在癌症发生的细胞增殖和死亡等多种情况中发挥着至关重要的作用。因此，通过纳米形式的实现这些生物标志物的调控，可能成为控制肿瘤进展的有前途的工具。利用硝酸银还原制备了纳米银粒子-聚（乙烯吡咯烷酮）（AgNPs-PVP），并以PVP稳定。通过产量百分比、UV-VIS、FT-IR、大小、电荷和形态进行表征。获得的AgNPs被用于抗前列腺癌（PC 3）和人类皮肤成纤维细胞（HFS）细胞系的抗癌活性测试。此外，还进行了基于生物标志物如TNF-α和IL-6的确认。合成的AgNPs-PVP稳定，呈球形状，粒径为122.33±17.61nm，聚散度指数为0.49±0.07，表面电荷为-19.23±0.61mV。体外细胞毒性测试显示，AgNPs-PVP表现出PC3细胞的抗增殖性质，并呈剂量依赖性。此外，与对照细胞相比，AgNPs-PVP的TNF-α较低且显著（∗p <0.05），分别为16.84±0.71pg/ml和20.81±0.44pg/ml。此外，HSF细胞的IL-6产生也显著降低（∗∗∗p <0.001）。本研究提示，AgNPs-PVP可能是一种治疗人类前列腺癌和癌细胞和非癌细胞的抗IL-6药物。将继续开展进一步研究以探究AgNPs-PVP在不同癌症类型中的效果。版权所有 © 2022 Ahmed A. H. Abdellatif等人。
Tumor necrosis factor (TNF-α) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers could be a promising tool for controlling tumor progression using nanoformulations. Silver nanoparticles-poly (vinyl pyrrolidone) (AgNPs-PVP) were prepared using the reduction of silver nitrate and stabilized with PVP. They are characterized through yield percentage, UV-VIS, FT-IR, size, charge, and morphology. The obtained AgNPs were tested for anticancer activity against prostate cancer (PC 3) and human skin fibroblast (HFS) cell lines. Moreover, biomarker-based confirmations like TNF-α and IL-6 were estimated. The synthesized AgNPs-PVP were stable, spherical in shape, with particle sizes of 122.33 ± 17.61 nm, a polydispersity index of 0.49 ± 0.07, and a negative surface charge of -19.23 ± 0.61 mV. In vitro cytotoxicity testing showed the AgNPs-PVP exhibited antiproliferation properties in PC3 in a dose-dependent manner. In addition, when compared to control cells, AgNPs-PVP has lower TNF-α with a significant value ( ∗ p < 0.05); the value reached 16.84 ± 0.71 pg/ml versus 20.81 ± 0.44 pg/ml, respectively. In addition, HSF cells showed a high level of reduction ( ∗∗∗ p < 0.001) in IL-6 production. This study suggested that AgNPs-PVP could be a possible therapeutic agent for human prostate cancer and anti-IL-6 in cancerous and noncancerous cells. Further studies will be performed to investigate the effect of AgNPs-PVP in different types of cancer.Copyright © 2022 Ahmed A. H. Abdellatif et al.