袋鼠对虾的微RNA已知可以促进肿瘤细胞凋亡，然而其具体机制尚不清楚。我们使用了能够检测细胞内部结构的层析显微镜观察了miRNA处理后的乳腺肿瘤细胞。有趣的是，我们发现线粒体通过一种名为隧道纳米管的结构迁移到相邻的肿瘤细胞中。为了重现这一过程，我们制造了一个微流控装置，将线粒体转移。我们展示了这种线粒体转移过程会将内切酶G（Endo G）释放到肿瘤细胞中，我们称之为未封闭线粒体。重要的是，单独的Endo G枯竭的线粒体没有抗肿瘤效果。此外，未封闭线粒体与亚毒性剂量的多柔比星具有协同的凋亡效应，从而减轻了心脏毒性。综上所述，我们展示了通过微流控技术进行的线粒体转移可以提供朝向肿瘤细胞死亡的潜在新策略。©2022年。作者。

Micro RNA of Marsupenaeus japonicas has been known to promote apoptosis of tumor cells. However, the detailed mechanisms are not well understood.Using tomographic microscope, which can detect the internal structure of cells, we observed breast tumor cells following treatment of the miRNA. Intriguingly, we found that mitochondria migrate to an adjacent tumor cells through a tunneling nanotube. To recapitulate this process, we engineered a microfluidic device through which mitochondria were transferred. We show that this mitochondrial transfer process released endonuclease G (Endo G) into tumor cells, which we referred to herein as unsealed mitochondria. Importantly, Endo G depleted mitochondria alone did not have tumoricidal effects. Moreover, unsealed mitochondria had synergistic apoptotic effects with subtoxic dose of doxorubicin thereby mitigating cardiotoxicity.Together, we show that the mitochondrial transfer through microfluidics can provide potential novel strategies towards tumor cell death.© 2022. The Author(s).