自然产物及其衍生物一直被视为传统合成分子的替代品，用于药物治疗，包括癌症化疗和具有抗衰老活性的化妆品成分。细胞衰老被认为是皮肤衰老的主要驱动因素，然而以特定方式针对皮肤衰老的天然产物并未得到彻底的探究。在这里，我们进行了一次专注化合物筛选，以识别具有抗衰老效应的天然产物。我们发现，靛蓝、靛蓝提取物在衰老的人类皮肤成纤维细胞上表现出抗衰老作用。此外，以靛蓝提取物治疗可以减轻与衰老相关的分泌表型（SASP）的炎症。有趣的是，靛蓝提取物对衰老的人类肺成纤维细胞只有微弱的细胞毒性作用。因此，我们的研究结果揭示了靛蓝提取物针对皮肤衰老的潜在活性，并建议靛蓝提取物是预防皮肤衰老的化妆品中具有吸引力的成分。

Natural products and their derivatives historically represent alternatives to conventional synthetic molecules for pharmacotherapy, ranging from cancer chemotherapeutics to cosmetic ingredients that exert anti-aging activities. Cellular senescence is considered a main driver of skin aging, yet natural products that target skin senescence in a specific manner are not thoroughly explored. Here, we performed a focused compound screen to identify natural products that exert anti-senescence effects. We found that Isatis tinctoria, woad extracts, displayed a senolytic effect on senescent human skin fibroblasts. Furthermore, treatment with woad extracts attenuated the expression of pro-inflammatory senescence-associated secretory phenotype (SASP), showing a senostatic activity. Intriguingly, woad extracts displayed only a marginal cytotoxic effect toward senescent human lung fibroblasts. Thus, our results reveal the potential activities of woad extracts for targeting skin senescence and suggest that woad extracts could be an attractive ingredient for cosmetics to prevent skin aging.© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.