全球范围内，女性乳腺癌是最常见的诊断癌症，是一个严重的全球健康威胁。传统的癌症治疗方法存在各种副作用，因此开发更好的乳腺癌治疗方法非常必要，例如激光治疗可能是一种有前途的治疗选择。本研究的目的是使用T47D细胞系作为体外模型，研究飞秒激光照射对乳腺癌的影响。将细胞以5×104个/孔的密度种植在96孔板中，并在过夜孵育后，将细胞暴露于不同波长（紫外线、可见光和红外线）的飞秒激光照射下，持续3、5或10分钟，并保持恒定的功率100毫瓦。使用MTT分析法直接测量细胞存活率，并在飞秒激光照射后24小时进行测量。在使用不同的飞秒激光照射参数时，特别是380和400纳米的飞秒激光照射，无论是直接还是在飞秒激光暴露后24小时，乳腺癌细胞生长均有显著抑制。同时，420和440纳米的飞秒激光也对细胞的存活率产生显著影响。另外，观察到延长暴露时间会增强观察到的效应，因此，10分钟的暴露时间是最好的。但是，700、720、750和780纳米的飞秒激光在不同的暴露时间内对细胞存活率没有显著影响。从上述结果可以得出结论，飞秒激光照射可对T47D细胞产生明显的抗癌效果。因此，飞秒激光可能成功应用于乳腺癌治疗。版权所有 © 2023 Elsevier B.V.。

Worldwide, the most frequently diagnosed cancer is female breast cancer, and it poses a serious global health threat. Traditional cancer therapies are associated with various side effects, so developing better therapies for breast cancer is necessary, such as laser therapy which could be a promising treatment option. The aim of the current study was to investigate the femtosecond laser irradiation effect on breast cancer using T47D cell line as an in vitro model. Cells were seeded at a density of 5 × 104 cells/well in 96-well plates and incubated overnight. After that, the cells were exposed to femtosecond laser irradiation at various wavelengths falling in the UV, visible, and IR ranges for 3, 5, or 10 min and at a constant power of 100 mW. Cell viability was measured directly and 24 h after femtosecond laser irradiation using MTT assay. When using different femtosecond laser irradiation parameters, especially the 380 and 400 nm femtosecond laser irradiation, there was significant inhibition of breast cancer cell growth, either directly or 24 h after femtosecond laser exposure. Also, 420 and 440 nm significantly affected the viability of the cells. It was also observed that increasing exposure time enhances the observed effect, so 10 min exposure time was the best time of exposure. However, 700, 720, 750, and 780 nm did not significantly affect the cells viability with different exposure times. It was possible to conclude from the aforementioned results that femtosecond laser irradiation exerted a significant anticancer effect against T47D cells. Consequently, the femtosecond laser could be used successfully for breast cancer management.Copyright © 2023 Elsevier B.V. All rights reserved.