卵巢癌是一种全球发病率逐年增加的疾病，迫切需要化学治疗和生物靶向治疗。上皮-间充质转化（EMT）是肿瘤细胞获得侵袭和转移能力的重要启动阶段。越来越多的发现表明，人类Schlafen家族成员5（SLFN5）在异常增殖中发挥着关键作用。然而，SLFN5在卵巢癌细胞中的作用尚未完全阐明。样本来自杭州市第一人民医院诊断为卵巢癌的患者，并通过荧光定量PCR检测SLFN5的表达。利用癌症基因组图谱（TCGA）数据库的表达谱数据，分析了SLFN5表达与卵巢癌进展和恶性程度的关系。利用荧光定量PCR研究了SLFN5相关上游和下游信号通路的mRNA表达水平。通过siRNA转染进行SLFN5沉默。通过Western blot检测SLFN5和相关转移蛋白的表达。Transwell和划痕愈合实验研究了卵巢癌细胞的迁移和侵袭能力。TCGA数据库分析结果显示，在高SLFN5表达人群中，与低SLFN5表达组相比，OS较差（P = 0.011）。SLFN5沉默对卵巢癌细胞EMT和侵袭运动具有显著的抑制作用。采用RT-PCR方法检测卵巢癌组织和邻近组织中SLFN5 mRNA的变化。结果发现，卵巢癌组织中SLFN5的表达增加，具有显著差异（P <0.05）。综上所述，这些结果表明SLFN5可能在卵巢癌细胞的肿瘤发生和发展中发挥协同作用，为未来卵巢癌治疗的药物开发提供了一个潜在靶点。 © 2023.作者

Ovarian cancer is a disease with increasing incidence worldwide, and there is an urgent need for chemotherapy and biological targeted therapy. Epithelial-mesenchymal transformation (EMT) is an important initiation stage for tumor cells to acquire the ability to invade and metastasize. A growing number of findings suggest that human Schlafen family member 5(SLFN5) plays a key role in malignancy. However, the role of SLFN5 in ovarian cancer cells has not been fully elucidated. Samples were collected from patients with ovarian cancer diagnosed in Hangzhou First People's Hospital, and the expression of SLFN5 was detected by fluorescence quantitative PCR. The relationship between SLFN5 expression and the progression and malignancy of ovarian cancer was analyzed by using the expression profile data from the Cancer Genome Atlas (TCGA) database. The mRNA expression levels of SLFN5 related upstream and downstream signaling pathways were studied by fluorescence quantitative PCR. Silencing SLFN5 was performed by siRNA transfection. The expression of SLFN5 and transfer-related proteins was examined by Western blot. Transwell and wound healing experiments investigated the migration and invasion ability of ovarian cancer cells. TCGA database analysis results showed that in the population with high SLFN5 expression, compared with the group with low SLFN5 expression, OS was worse (P = 0.011). SLFN5 silencing had a significant inhibitory effect on EMT and invasion movement of ovarian cancer cells. RT-PCR method was used to detect the mRNA changes of SLFN5 in ovarian cancer tissue and adjacent tissue. It was found that the expression of SLFN5 in ovarian cancer tissue was increased, with a significant difference (P < 0.05). Together, these results suggest that SLFN5 may play a synergistic role in tumorigenesis and development of ovarian cancer cells, providing a potential target for future drug development for the treatment of ovarian cancer.© 2023. The Author(s).