生物正交配合物联结活细胞与基底或其他细胞需要高稳定性和快速动力学来保持目标细胞的本质。在这项研究中，我们报道了水溶性环八二炔（WS-CODY）衍生物，其经历离子对增强的双击反应。由于近距离效应，WS-CODY的阳离子侧链加速了偶氮丙酰修饰的细胞表面的动力学。阳离子WS-CODY能够将偶氮修饰的、粘附性较差的人肺癌PC-9细胞与偶氮嫁接的玻璃基底以及其他细胞交联，时间仅需5-30分钟。我们发现细胞-基质交联诱导ITGA5基因表达，而细胞-细胞交联则根据粘附伴侣诱导CTNNA1基因表达。离子对增强型WS-CODY可应用于已建立偶氮修饰的各种细胞，预计将成为调节细胞-基质和细胞-细胞相互作用的强大工具。

Bio-orthogonal ligations that crosslink living cells with a substrate or other cells require high stability and rapid kinetics to maintain the nature of target cells. In this study, we report water-soluble cyclooctadiyne (WS-CODY) derivatives that undergo an ion-pair enhanced double-click reaction. The cationic side chain of WS-CODY accelerated the kinetics on the azide-modified cell surface due to proximity effect. Cationic WS-CODY was able to crosslink azide-modified, poorly adherent human lung cancer PC-9 cells not only to azide-grafted glass substrates but also to other cells within 5-30 min. We discovered that cell-substrate crosslinking induced the ITGA5 gene expression, whereas cell-cell crosslinking induced the CTNNA1 gene, according to the adhesion partner. Ion-pair-enhanced WS-CODY can be applied to a wide range of cells with established azide modifications and is expected to provide a powerful tool to regulate cell-substrate and cell-cell interactions.