初步研究表明，相对于非动脉粥样硬化冠状动脉组织（NCAT）样本，低密度脂蛋白受体（LDLR）、肿瘤蛋白（TP53）和基质金属蛋白酶 9（MMP9）基因表达水平在动脉粥样硬化冠状动脉组织（ACAT）中明显增加。因此，进行了进一步研究以研究LDLR、TP53和MMP9基因多态性与马来西亚人群动脉粥样硬化（ATH）发病风险的关联。在76个ACAT样本和149个NCAT样本中分别分别代表病例和对照组分析了C88S、 TP53密码子72和MMP9C>T的单核苷酸多态性。结果表明，MMP9C>T多态性的杂合CT基因型在ACAT中明显高于NCAT样本（57.9% vs. 27.5%，χ2=19.758，df=1，p<0.05）。CT基因型被发现与发展ATH的风险显著相关（OR=3.622，95% CI=2.028-6.470）。然而，无论性别和种族如何，健康的马来西亚人群中CT基因型的分布都是不可比的。DNA测序结果验证了C88S、TP53密码子72和MMP9C>T的多态性。总之，MMP9-1562C>T多态性的CT基因型被发现与发展ATH的风险强烈相关。版权所有©2023 Elsevier Inc.发表。

Preliminary research has shown that low density lipoprotein receptor (LDLR), tumor protein (TP53) and matrix metalloproteinase 9 (MMP9) genes expression levels were significantly increased in atherosclerosis coronary artery tissue (ACAT) compared to non-atherosclerotic coronary artery tissue (NCAT) samples. Thus, further investigation was carried out to study the association of LDLR, TP53 and MMP9 gene polymorphisms and the risk of developing atherosclerosis (ATH) in a Malaysian population. Single nucleotide polymorphisms of C88S, TP53 codon 72 and MMP9C>T were analyzed in 76 ACAT samples and 149 NCAT samples, representing cases and controls, respectively. In results, heterozygous CT genotype of MMP9C>T polymorphism was significantly higher in ACAT compared to NCAT samples (57.9% vs. 27.5%, χ2 = 19.758, df= 1, p < 0.05). The CT genotype was found to be significantly associated with the risk of developing ATH (OR = 3.622, 95% CI = 2.028-6.470). However, the distribution of the CT genotype in a healthy Malaysian study population was incomparable regardless of gender and ethnicity. The DNA sequencing results validated the C88S, TP53 codon 72, and MMP9C>T polymorphisms. In conclusion, the CT genotype of the MMP9-1562C>T polymorphism was found to have a strong association with the risk of developing ATH.Copyright © 2023. Published by Elsevier Inc.