微藻生产众多的一级代谢物和二级代谢物，在多个市场部门中可能具有应用，包括化妆品、人类营养、水产养殖、生物柴油生产以及治疗/预防人类疾病。其中硅藻是最多样化的微藻类群，其中许多物种已知具有抗癌、抗氧化、抗糖尿病、抗炎和免疫调节等特性。这些活性化合物的成分通常仍然未知。本研究旨在对从两个不同位置采样的两株星尾链藻进行全转录组的进行去 novo 序列化，并在对照组和磷饥饿条件下培养。我们采用 RNA-seq 方法来寻找与合成/降解具有抗癌和免疫调节特性化合物的转录本。我们鉴定了编码 L-天门冬酰胺酶 I、多酮类环合酶/脱水酶、双功能多酮磷酸酶/激酶、1-脱氧-D-木糖-5-磷酸合酶（片段）、肌醇多磷酸酶 INPP5B/F、儿茶酚-O-甲基转移酶、二半乳糖基二酰基甘油合成酶（DGD1）、1,2-二酰基甘油-3-β-半乳糖苷基转移酶和甘油磷酸二酯磷酸二酯酶的转录本。差异表达分析还可以确定这些酶在哪种培养条件下更加表达。总的来说，这些数据为硅藻基因、生物活性分子生成所涉及的酶途径的注释以及星尾链藻的可能利用提供了新的见解。

Microalgae produce a plethora of primary and secondary metabolites with possible applications in several market sectors, including cosmetics, human nutrition, aquaculture, biodiesel production and treatment/prevention of human diseases. Diatoms, in particular, are the most diversified microalgal group, many species of which are known to have anti-cancer, anti-oxidant, anti-diabetes, anti-inflammatory and immunomodulatory properties. Compounds responsible for these activities are often still unknown. The aim of this study was to de novo sequence the full transcriptome of two strains of the diatom Asterionellopsis thurstonii, sampled from two different locations and cultured in both control and phosphate starvation conditions. We used an RNA-sequencing approach to in silico identify transcripts potentially involved in the synthesis/degradation of compounds with anti-cancer and immunomodulatory properties. We identified transcript coding for L-asparaginase I, polyketide cyclase/dehydrase, bifunctional polyketide phosphatase/kinase, 1-deoxy-D-xylulose-5-phosphate synthase (fragment), inositol polyphosphate 5-phosphatase INPP5B/F, catechol O-Methyltransferase, digalactosyldiacylglycerol synthase (DGD1), 1,2-diacylglycerol-3-beta-galactosyltransferase and glycerolphosphodiester phosphodiesterase. Differential expression analysis also allowed to identify in which culturing condition these enzymes are more expressed. Overall, these data give new insights on the annotation of diatom genes, enzymatic pathways involved in the generation of bioactive molecules and possible exploitation of Asterionellopsis thurstonii.