Dipsaci Radix（DR）是裂叶石斛的干燥根，具有滋肝肾、强筋骨、调节血管等作用，但其主要活性成分仍知之甚少。本研究旨在通过光谱-效应关系，找到DR治疗骨关节炎（OA）的主要活性成分，并比较RDR和WDR的差异。首先，采用高效液相色谱法（HPLC）建立了DR的指纹图谱，并使用UPLC-Q-TOF/MS确定了其中的10个峰。然后，通过注射碘乙酸钠建立OA大鼠模型，研究DR对OA的影响。光谱-效应关系采用灰色关联分析（GRA）和皮尔森相关分析进行分析。根据药理结果，与模型组相比，低、中、高剂量组大鼠的软骨评分、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）和曼金评分均降低，而葡萄酒处理DR的治疗效果在相同剂量下趋于更好。最后，通过GRA和皮尔森相关分析，初步确定了DR的活性成分为4（对数酸）、6（绿原酸）、8（咖啡酸）、14（裂叶石斛苷B）、16和17（裂叶石斛皂苷VI）。本研究首次建立了原料和葡萄酒处理DR之间的光谱-效应关系，为研究DR加工前后的药效物质基础提供了理论依据，并为DR的进一步研究提供了参考。 版权所有 ©2023 Elsevier B.V.发行。

Dipsaci Radix (DR) is the dry root of the Dipsacus asper Wall. ex DC., which has the function of tonifying the liver and kidney, continuing tendons and bones, and regulating blood vessels. However, there are few reports on the main active ingredients.This study aimed to find the main active components of DR in the treatment of osteoarthritis (OA) by spectrum-effect relationship and compare the differences between RDR and WDR.Firstly, the high-performance liquid chromatography (HPLC) method was used to establish the fingerprint of DR, and 10 peaks of them were determined by UPLC-Q-TOF/MS. Then, the OA rat model was established by injecting sodium iodoacetate to study the effect of DR on OA. The spectrum-effect relationship was analyzed by grey relational analysis (GRA) and Pearson correlation analysis.According to the pharmacological results, compared with the model group, the cartilage score, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), and Mankin score of rats in low, medium and high dose groups were decreased, and the therapeutic effect of wine-processed DR tended to be better than raw DR at the same dose. Finally, the active components of DR were preliminarily determined as 4 (loganic acid), 6 (chlorogenic acid), 8 (caffeic acid), 14 (dipsanoside B), 16, and 17 (asperosaponin VI) which had a large correlation in GRA and Pearson correlation analysis.This study established the spectrum-effect relationship between the raw and wine-processed DR for the first time, which provided a theoretical basis for the study of the pharmacodynamic substance basis of DR before and after processing. This research provided a reference for the subsequent study of DR.Copyright © 2023. Published by Elsevier B.V.