上皮间充质转化 (EMT) 是一个持续的过程，包括上皮、部分 EMT 和间充质状态，每种状态都与癌症进展、侵袭性能力以及最终转移有关。我们使用胰腺癌随机分化模型来生成小鼠器官样本库，包括经历部分 EMT 和完全 EMT 的分支系。通过一种无偏的蛋白组学方法，我们发现器官样本形态预测了 EMT 状态，实体器官样本和部分 EMT 签名有关。我们还观察到外源性 TGFβ1 诱导实体器官样本形态，与 S100 家族的变化、完全 EMT 和高级别肿瘤的形成有关。S100A4 或许是预测胰腺癌患者 EMT 状态、疾病进展和结果的有用生物标记物。© 2023 作者。

Epithelial-mesenchymal transition (EMT) is a continuum that includes epithelial, partial EMT, and mesenchymal states, each of which is associated with cancer progression, invasive capabilities, and ultimately, metastasis. We used a lineage-traced sporadic model of pancreatic cancer to generate a murine organoid biobank from primary and secondary tumors, including sublines that underwent partial EMT and complete EMT. Using an unbiased proteomics approach, we found that organoid morphology predicts the EMT state, and the solid organoids are associated with a partial EMT signature. We also observed that exogenous TGFβ1 induces solid organoid morphology that is associated with changes in the S100 family, complete EMT, and the formation of high-grade tumors. S100A4 may be a useful biomarker for predicting EMT state, disease progression, and outcome in patients with pancreatic cancer.© 2023. The Author(s).