研究动态
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从蓝藻菌群中提取的多糖的生物潜力,用于制作膜的物理和生物特性。

Biological potential of polysaccharides extracted from Nostoc colonies for film production ࣧ Physical and biological properties.

发表日期:2023 Feb 25
作者: Joanna Maria Jasińska, Iwona Kamińska, Ewelina Jamróz, Maria J Chmiel
来源: Biotechnology Journal

摘要:

Nostoc属的蓝藻分泌一些生物活性化合物,包括多糖,可能具有抗氧化、抗微生物、抗癌和抗炎作用。本研究旨在研究Nostoc多糖(NPs)的生物性质(抗氧化和抗微生物)以及在生物膜生产中添加NPs的可能性。我们的结果表明,Nostoc多糖与使用的生物聚合物基质(氧化冰洁和壳聚糖)相容,并表现出抗氧化性。测试的多糖提取物(0.14%)在ABTS和DPPH水平上表现出中和自由基的能力分别为4.46%和10.14%。 NP提取物表现出15.35和30.07 mg Trolox当量(FRAP和CUPRAC方法,分别)和2.64 mg绿原酸当量(使用Folin's试剂测试)的还原特性。NP提取物对测试的微生物表现出生长刺激效应(大肠杆菌,金黄色葡萄球菌,酿酒酵母),对青霉菌没有影响或轻微的抑制作用(链霉菌)。向氧化冰洁和壳聚糖膜中添加多糖会影响所得生物膜的物理化学特性。与对照相比,将多糖添加到氧化冰洁和壳聚糖膜中会降低其溶解性(分别降低约40%和9%),同时增加其吸水性。本文受版权保护。版权所有。
Cyanobacteria of the Nostocgenus secrete a number of biologically active compounds, including polysaccharides, which may exhibit antioxidant, antimicrobial, anticancer and anti-inflammatory properties. The aim of the study was to investigate the biological properties of Nostoc polysaccharides (NPs) (antioxidative and antimicrobial) and the possibility of using NPs addition in the production of biofilms.Our results allow to indicate that Nostoc polysaccharides were compatible with the used biopolymer matrix (furcellaran and chitosan) and showed antioxidant properties. The tested polysaccharide extracts (0.14%) exhibited the ability to neutralise free radicalsࣧ ABTS and DPPH at a level of 4.46% and 10.14%, respectively. NP extracts demonstrated reducing properties of 15.35 and 30.07 mg Trolox equivalents (FRAP and CUPRAC methods, respectively) and 2.64 mg chlorogenic acid equivalents (tested with Folin's reagent). NP extracts showed: a growth-stimulating effect (E. coli, S. aureus, S. cerevisiae), no effect (Penicillum sp.) or a slight inhibitory effect (Streptomyces sp.) on the tested microorganisms. The enrichment of the film with NPs influenced the physic-chemical properties of the obtained biofilms. The addition of polysaccharides to furcellaran and chitosan films decreased their water solubility (by approximately40% and 9%, respectively, compared to the control) and, at the same time increased, their water absorption. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.