植物源類似療法藥物 (HMs) 便宜易得，但其機制較傳統藥物少有探討。本研究旨在評估從刺楸 (BA)、懸鉤子 (BV)、薄荷 (MP)、薑黃 (CL)、金鎖樹 (CO)、西洋杉 (TO) 和金縷梅 (HC) 等植物中提取的HMs對子宮頸癌 (CaCx) 細胞的影響。我們通過MT和30C HMs在MTT assays下篩選上述HMs的抗增殖和細胞毒性活性對HPV陰性（C33a）和陽性CaCx細胞（SiHa和HeLa）進行測試。還採用標準化的測試方法測定了每種HM的總多酚含量 (TPC) 和自由基清除活性。為檢測這些HMs是否有潛在的抑制HPV16 E6的能力，我們還對這些HMs中據報可獲得的植物化學物質進行了分子對接研究。 所有測試的MTs引起了不同的、劑量依賴的細胞毒性反應，這種反應隨細胞系的不同而有所變化。對於C33a細胞，反應順序是TO > CL > BA > BV > HC > MP > CO，而對於SiHa和HeLa細胞，反應順序分別是HC > MP > TO > CO > BA > BV > CL和CL > BA > CO。所有HMs的30C HMs的反應不一致。此外，MTs所表現的抗CaCx反應並不遵循HM的多酚含量或自由基清除能力的順序。分析顯示，BA、BV和HC的抗氧化物含量對其抗CaCx活性的貢獻最低。透過對BV、BA、HC、CL和TO的主要植物化學成分與HPV16 E6蛋白質晶體結構 (6SJA和4XR8) 的分子對接進行理論分析，確定了它們抑制HPV16 E6蛋白質致癌反應的潛力。 本研究首次對多種植物源MT和HMs的潛力進行了細胞毒性和自由基清除的比較評估，並首次開展了它們在理論分子水平上的潛在分子靶標研究。數據表明，BA和BV的MTs可能是最強大的HMs，能夠強烈抑制CaCx的生長，並具有強大的抗HPV植物化學成分。

 Plant-derived homeopathic medicines (HMs) are cheap and commercially available but are mechanistically less explored entities than conventional medicines. The aim of our study was to evaluate the impact of selected plant-derived HMs derived from Berberis aquifolium (BA), Berberis vulgaris (BV), Mentha piperita (MP), Curcuma longa (CL), Cinchona officinalis (CO), Thuja occidentalis (TO) and Hydrastis canadensis (HC) on cervical cancer (CaCx) cells in vitro. We screened the mother tincture (MT) and 30C potencies of the above-mentioned HMs for anti-proliferative and cytotoxic activity on human papillomavirus (HPV)-negative (C33a) and HPV-positive CaCx cells (SiHa and HeLa) by MTT assay. Total phenolic content (TPC) and the free-radical scavenging activity of each HM was also determined using standard assays. Phytochemicals reportedly available in these HMs were examined for their potential inhibitory action on HPV16 E6 by in silico molecular docking. All tested MTs induced a differential dose-dependent cytotoxic response that varied with cell line. For C33a cells, the order of response was TO > CL > BA > BV > HC > MP > CO, whereas for SiHa and HeLa cells the order was HC > MP > TO > CO > BA > BV > CL and CL > BA > CO, respectively. 30C potencies of all HMs showed an inconsistent response. Further, anti-CaCx responses displayed by MTs did not follow the order of an HM's phenolic content or free radical scavenging activity. Analysis revealed anti-oxidant content of BA, BV and HC had the lowest contribution to their anti-CaCx activity. Using in silico modeling of molecular docking between the HPV16 E6 protein crystallographic structures (6SJA and 4XR8) and main phytochemical components of BV, BA, HC, CL and TO, their potential to inhibit the HPV16 E6 protein carcinogenic interactions was identified. The study has shown a comparative evaluation of the potential of several plant-derived MTs and HMs to affect CaCx cell line survival in vitro (through cytotoxicity and free radical scavenging) and their theoretical molecular targets in silico for the first time. Data demonstrated that MTs of BA and BV are likely to be the most potent HMs that strongly inhibited CaCx growth and have a strong anti-HPV phytochemical constitution.Faculty of Homeopathy. This article is published by Thieme.