结肠癌（CRC）是世界范围内第二大死因和第三大确诊癌症。尽管姜黄素（CUR）表现出很强的抗癌活性，但其特点是溶解性差、生物利用度低和不稳定性。本研究是前期调查的延伸，其中CUR和琥珀酸丙酰化CUR（CUR.SA）分别被封装在甘露糖基壳聚糖纳米粒子（CM-NPs）中形成CUR-NPs和CUR.SA-NPs。我们旨在评估这两种纳米制剂的抗CRC活性。CCK-8试验使用细胞毒性研究表明，CUR-NPs和CUR.SA-NPs对CRC人细胞系（HCT116和SW480）具有剂量和时间依赖性毒性，且比自由CUR或CUR-SA更具细胞毒性。观察到CUR-NPs和CUR.SA-NPs处理的CRC细胞系中早期和晚期凋亡的显著诱导。Western blot分析证实了通过激活Caspase信号途径诱导凋亡和未处理细胞的区别。基于CUR-NPs和CUR.SA-NPs的物理化学性质以及体外研究数据，我们可以得出结论，这些纳米颗粒制剂拥有非常有前途的属性，值得进一步研究其在CRC管理中的作用。版权所有 © 2023. Elsevier B.V. 发布。

Colon cancer (CRC) is the second leading cause of death and the third most diagnosed cancer worldwide. Although curcumin (CUR) has demonstrated a potent anticancer activity, it is characterized by its poor solubility, low bioavailability, and instability. This study is a projection from a previous investigation where CUR and succinylated CUR (CUR.SA) were separately encapsulated in mannosylated-chitosan nanoparticles (CM-NPs) to form CUR-NPs and CUR.SA-NPs, respectively. Here, we aim to assess the anti-CRC activity of these two nanoformulations. Cytotoxicity studies using CCK-8 assay indicated that both CUR-NPs and CUR.SA-NPs have a dose and time-dependent toxicity towards CRC human cell-lines (HCT116 and SW480), and more cytotoxic compared to free CUR or CUR-SA in a time-dependent manner. A significant induction of early and late apoptosis in the CUR-NPs and CUR.SA-NPs treated CRC cell lines compared to untreated cells was observed. Western blotting analyses confirmed the induction of apoptosis through activation of Caspase signaling compared to untreated cells. Based on the physicochemical properties of CUR-NPs and CUR.SA-NPs along with the data from the in vitro studies, we may conclude these nanoparticle formulations hold very promising attributes, worthy of further investigations for its role in the management of CRC.Copyright © 2023. Published by Elsevier B.V.