本研究旨在结合单细胞RNA测序（scRNA-seq）与网络药理学，探讨川产白花蛇舌草（HDW）和鸭距草（SB）在结直肠癌（ccRCC）中的治疗机制。通过中药系统药理学数据库和分析平台获取了HDW-SB的活性成分和潜在分子靶点。从基因表达组学数据库Gene Expression Omnibus取得了基因表达数据（GSE53757）。通过蛋白质相互作用网络确定了HDW-SB在对抗ccRCC时的核心基因，并通过分子复合物检测进一步分析了这些基因。分析了这些基因在ccRCC的诊断和免疫浸润中的作用。利用单细胞RNA测序数据（GSE121638）和分子对接验证了这些核心基因的临床意义。蛋白质相互作用网络分析后，鉴定出HDW-SB与ccRCC相对抗的29个核心基因。除了CENPE外，所有核心基因在肿瘤组织中的表达均有显著差异，并更准确地诊断了ccRCC。根据单细胞RNA测序数据集，定义了15个细胞簇，并根据标记基因将这些簇注释为6个细胞类型。核心基因中的TYMS和KIAA0101在NK细胞中高表达。从化合物-靶点相互作用网络中发现，黄酮、黄酮醇和黄芩素等三种活性化合物靶向TYMS和KIAA0101。鉴定出HDW-SB与ccRCC相对抗的29个核心基因，并在诊断和预后方面表现出良好性能。而且，在这些核心基因与HDW-SB的主要成分进行对接时，TYMS和KIAA0101通过NK细胞发挥了抗ccRCC的作用。Copyright© Bentham Science Publishers;若有任何疑问，请发送邮件至epub@benthamscience.net。

The present study aimed to investigate the therapeutic mechanism of Hedyotis diffusa Willd (HDW) and Scutellaria barbata (SB) in ccRCC using a combination of single-cell RNA sequencing (scRNA-seq) and network pharmacology.The active ingredients and potential molecular targets of HDW-SB were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. Gene expression data (GSE53757) were obtained from the Gene Expression Omnibus database. The hub genes of HDW-SB against ccRCC were identified via the protein-protein interaction network, and further analyzed by molecular complex detection. The roles of these genes in the diagnosis and immune infiltration of ccRCC were analyzed. The clinical significance of hub genes was verified using scRNA-seq data (GSE121638) and molecular docking.Following the PPI network analysis, 29 hub genes of HDW-SB against ccRCC were identified. All hub genes, except for CENPE, had significantly different expressions in tumor tissue and a more accurate diagnosis of ccRCC. Fifteen cell clusters were defined based on the scRNA-seq dataset, and the clusters were annotated as six cell types using marker genes. TYMS and KIAA0101 from hub genes were highly expressed in NK cells. Three active compounds, quercetin, luteolin, and baicalein, were found to target TYMS and KIAA0101 from the compound-target interaction network.29 hub genes of HDW-SB against ccRCC were identified and showed good performance in terms of diagnosis and prognosis. Moreover, among these hub genes docking with the main ingredients of HDW-SB, TYMS and KIAA0101 exerted anti-ccRCC effects through NK cells.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.