本研究旨在开发一种临床兼容的系统，以比传统的组织病理学评估更高效的方式进行乳腺组织分析。研究调查了该系统在手术室或外科手术室内应用于体内，以改善患者结局的潜力。在本研究中，使用了80对侵袭性导管癌和相邻正常乳腺组织的组织样本，在光时间解析荧光和弥散反射（DA）系统中进行测量。测量完成后，提取了胶原蛋白和黄素腺嘌呤二核苷酸（FAD）的荧光强度，胶原蛋白、尼克酰胺腺嘌呤二核苷酸（NADH）和FAD的荧光寿命，DA，吸收系数和还原散射系数。样本随后经历了组织学处理和H&E染色，以将其分类为肿瘤、纤维腺体和/或脂肪组织。不同组织群之间的胶原蛋白和FAD荧光强度、胶原蛋白和FAD荧光寿命、DA和散射系数之间存在明显的统计学差异。NADH荧光寿命和吸收系数在肿瘤和纤维腺体组之间、肿瘤和脂肪组之间也存在统计学差异。虽然许多乳腺组织分析研究将纤维腺体和脂肪组织合并标记为“正常”乳腺组织，但本研究表明，肿瘤和纤维腺体组织之间的某些差异与肿瘤和脂肪组织之间的差异并不相同。减少的散射系数的观察结果还可能表明进一步分类，包括纤维脂肪组织的分类可能是必要的。未来的工作将受益于额外的组织分类。由于三种组织类型之间的光学参数存在可观测的差异，本系统在临床环境中作为乳腺分析工具具有潜力。通过进一步涉及混合组织样本的工作，该组合系统有可能被用于术中快速边缘评估。 © 2023 版权所有。由Wiley Periodicals LLC出版的《激光外科与医学》上发表的文章。

This work aims to develop a clinically compatible system that can perform breast tissue analysis in a more time efficient process than conventional histopathological assessment. The potential for such a system to be used in vivo in the operating room or surgical suite to improve patient outcome is investigated.In this work, 80 matched pairs of invasive ductal carcinoma and adjacent normal breast tissue were measured in a combined time-resolved fluorescence and diffuse reflectance (DA) system. Following measurement, the fluorescence intensity of collagen and flavin adenine dinucleotide (FAD); the fluorescence lifetime of collagen, nicotinamide adenine dinucleotide (NADH), and FAD; the DA; absorption coefficient; and reduced scattering coefficient were extracted. Samples then underwent histological processing and H&E staining to classify composition as tumor, fibroglandular, and/or adipose tissue.Statistically significant differences in the collagen and FAD fluorescence intensity, collagen and FAD fluorescence lifetime, DA, and scattering coefficient were found between each tissue group. The NADH fluorescence lifetime and absorption coefficient were statistically different between the tumor and fibroglandular groups, and the tumor and adipose groups. While many breast tissue analysis studies label fibroglandular and adipose together as "normal" breast tissue, this work indicates that some differences between tumor and fibroglandular tissue are not the same as differences between tumor and adipose tissue. Observations of the reduced scatter coefficient may also indicate further classification to include fibro-adipose may be necessary. Future work would benefit from the additional tissue classification.With observable differences in optical parameters between the three tissue types, this system shows promise as a breast analysis tool in a clinical setting. With further work involving samples of mixed composition, this combined system could potentially be used intraoperatively for rapid margin assessment.© 2023 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.