本研究利用一株从自然发酵泡菜中分离的植物乳杆菌CQPC02（LP-CQPC02）进行实验。为构建狼疮肾炎的动物模型，我们使用朴赛混合油。随后，我们采用试剂盒鉴定小鼠血液和组织中的标志物，并使用定量聚合酶链式反应（qPCR）测量小鼠肾组织中与核因子κ-B（NF-κB）相关的基因表达。根据实验结果，经LP-CQPC02口服可能减轻狼疮肾炎所致的尿蛋白升高，以及血清和肾脏组织中炎症细胞因子水平，包括白细胞介素6（IL-6）、白细胞介素12（IL-12）、肿瘤坏死因子α和干扰素。此外，在肾炎小鼠中，LP-CQPC02可降低血清肌酐（SCr）、血尿素氮（BUN）、总胆固醇（TC）、甘油三酯（TG）的水平，并提高总蛋白（TP）和白蛋白（ALB）的水平。在肾炎小鼠中，LP-CQPC02还能够降低双链脱氧核酸（dsDNA）的阳性率。病理切片观察显示，LP-CQPC02可以减轻肾炎导致的肾小球形态不完整和炎性浸润等组织损伤。在狼疮肾炎小鼠的肾脏中，LP-CQPC02能够上调核因子κ-B抑制因子（IκB-α）的表达，下调NF-κB、转化生长因子β1（TGF-β1）、血管内皮生长因子（VEGF）、细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达。已证实植物乳杆菌CQPC02对小鼠肾炎具有干预效果，并具有作为益生菌的潜力。© 2023. 作者以及该研究的授权人 Sociedade Brasileira de Microbiologia。

A strain of Lactobacillus plantarum CQPC02 (LP-CQPC02) isolated from naturally fermented kimchi was utilized in this investigation. In order to construct an animal model of lupus nephritis, pristane was used. We then used a kit to identify markers in mouse blood and tissues and a quantitative polymerase chain reaction (qPCR) to measure the expression of genes associated to nuclear factor kappa-B (NF-κB) in mouse kidney tissue. According to the results of the experiments, oral administration of LP-CQPC02 LP-CQPC02 may lessen the lupus nephritis-related rise in urine protein as well as the cytokine levels that were rising in the serum and renal tissues, including IL-6, IL-12, tumor necrosis factor alpha, and interferon. Additionally, in mice with nephritis, LP-CQPC02 can lower serum creatinine (SCr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), and raise total protein (TP) and albumin (ALB) levels. In mice with nephritis, LP-CQPC02 can also reduce the positive rate of double-stranded deoxyribonucleic acid (dsDNA). Pathological sections were examined, and it was shown that LP-CQPC02 can lessen tissue damage such incomplete glomerular morphology and inflammatory infiltration brought on by nephritis. In the kidneys of mice with lupus nephritis, LP-CQPC02 can upregulate the expression of inhibitor of NF-κB (IκB-α), downregulate the expression of NF-κB, transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), intercellular cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). Lactobacillus plantarum CQPC02 has been confirmed to have an intervention effect on nephritis in mice and has the potential as a probiotic.© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.