用于监测体外人类心脏器官样体跳动的双模成像系统。
Dual-modality imaging system for monitoring human heart organoids beating in vitro.
发表日期：2023 Aug 01
作者：
Senyue Hao, Chao Ren, Fei Wang, Kibeom Park, Brett D Volmert, Aitor Aguirre, Chao Zhou
来源：
HEART & LUNG
摘要：
为了揭示人类心脏器官样体 (hHOs) 的三维微结构和钙动力学特征，我们开发了一个将光学相干断层扫描 (OCT) 和荧光显微镜优势结合的双模式成像系统。OCT 提供了高分辨率的体积结构信息，而荧光显微镜则显示了hHOs搏动行为的电生理学特征。我们验证了同时进行 OCT M-模式和钙成像能够从心脏器官样体中得到相同的搏动模式。我们进一步应用动态对比度 OCT (DyC-OCT) 分析来加强验证，并定位hHOs内的搏动聚集区。这种成像平台为在体外研究和评估hHOs提供了一种强大的工具，并具有疾病模型和药物筛选的潜在应用。
To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids (hHOs), we developed a dual-modality imaging system combining the advantages of optical coherence tomography (OCT) and fluorescence microscopy. OCT provides high-resolution volumetric structural information, while fluorescence imaging indicates the electrophysiology of the hHOs' beating behavior. We verified that concurrent OCT motion mode (M-mode) and calcium imaging retrieved the same beating pattern from the heart organoids. We further applied dynamic contrast OCT (DyC-OCT) analysis to strengthen the verification and localize the beating clusters inside the hHOs. This imaging platform provides a powerful tool for studying and assessing hHOs in vitro, with potential applications in disease modeling and drug screening.