三维组织电泳在多种肿瘤标本中用于高通量癌症边缘检测。
Three-Dimensional Histological Electrophoresis for High-Throughput Cancer Margin Detection in Multiple Types of Tumor Specimens.
发表日期：2023 Aug 01
作者：
Feiran Zhang, Jiajun Xu, Chengbin Zhang, Yin Li, Jiawei Gao, Limei Qu, Songling Zhang, Shoujun Zhu, Junhu Zhang, Bai Yang
来源：
MEDICINE & SCIENCE IN SPORTS & EXERCISE
摘要：
癌症手术中准确鉴定肿瘤边缘依赖于一种快速检测技术，该技术可以同时进行多个可疑肿瘤病灶的高通量检测。然而，冷冻组织切片的常规组织病理学分析，被视为黄金标准，在许多地区没有足够接受过培训的病理学家的情况下，往往会出现显著的变异。因此，在手术切除组织内，对肿瘤边缘进行准确和高通量评估的临床需求更迫切。我们在这里描述了一种高通量的三维（3D）组织电泳设备，该设备利用肿瘤特异性蛋白质在术中识别和勾画肿瘤边缘。对七个细胞系异种移植模型和人类宫颈癌模型（代表五种组织类型）的测试证实了此方法的高通量检测功能。我们预计，这种3D组织电泳设备将提高广泛的癌症诊断的准确性和效率。
Accurate identification of tumor margins during cancer surgeries relies on a rapid detection technique that can perform high-throughput detection of multiple suspected tumor lesions at the same time. Unfortunately, the conventional histopathological analysis of frozen tissue sections, which is considered the gold standard, often demonstrates considerable variability, especially in many regions without adequate access to trained pathologists. Therefore, there is a clinical need for a multitumor-suitable complementary tool that can accurately and high-throughput assess tumor margins in every direction within the surgically resected tissue. We herein describe a high-throughput three-dimensional (3D) histological electrophoresis device that uses tumor-specific proteins to identify and contour tumor margins intraoperatively. Testing on seven cell-line xenograft models and human cervical cancer models (representing five types of tissues) demonstrated the high-throughput detection utility of this approach. We anticipate that the 3D histological electrophoresis device will improve the accuracy and efficiency of diagnosing a wide range of cancers.