基于微量血液中乳腺癌循环肿瘤细胞的微流体分离。
Microfluidic isolation of breast cancer circulating tumor cells from microvolumes of mouse blood.
发表日期：2023 Aug 02
作者：
Celine Macaraniag, Jian Zhou, Jing Li, William Putzbach, Nissim Hay, Ian Papautsky
来源：
Disease Models & Mechanisms
摘要：
液体活检在癌症研究和临床中显示出重要的研究和临床意义。尤其是在临床前研究中，循环肿瘤细胞（CTCs）的分离可以提供关于疾病进展的关键信息，从而有助于指导治疗决策。微流体分离系统在癌症研究、疾病诊断和预后中起到了重要的作用。CTCs通常使用异种移植模型或同基因模型等临床前动物模型进行研究。然而，大多数分离系统是在人体细胞系和人血液上进行测试的，而对于小鼠模型中CTCs的验证研究较少。在这里，我们展示并评估了一种基于尺寸的惯性微流体装置的完整工作流程，仅使用小鼠血液和小鼠癌细胞系来分离CTCs。然后，我们结合细胞离心技术，这是一种常用的方法，在玻璃片上计数少量细胞，以量化我们工作流程的总细胞产量。© 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH.
Liquid biopsy has shown significant research and clinical implications in cancer. Particularly, the isolation of circulating tumor cells (CTCs) in preclinical studies can provide crucial information about disease progression and therefore may guide treatment decisions. Microfluidic isolation systems have played a considerable role in CTC isolation for cancer studies, disease diagnosis, and prognosis. CTCs are often studied using preclinical animal models such as xenografts or syngeneic models. However, most isolation systems are tested on human cell lines and human blood, whereas less validation studies are done on preclinical samples such as CTCs from mouse models. Here, we demonstrate and evaluate a complete workflow of a sized-based inertial microfluidic device to isolate CTCs from blood using exclusively mouse blood and mouse cancer cell lines. We then incorporate the cytospin, a commonly used method for enumeration of small number of cells in a glass slide to quantify the total cell yield of our workflow.© 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH.