氧化应激是由活性氧物质和自由基引起的，加速炎症反应并加重疲劳。罗汀酸（TA）具有抗氧化和抗炎性能。因此，本研究的目的是确定TA对H2O2刺激的肌肉前体细胞系C2C12细胞和踏车负荷试验（TST）和强迫游泳试验（FST）动物模型中的疲劳调节效应。在体外研究中，在刺激H2O2之前，C2C12细胞被预先用TA处理。然后，分析丙二醛（MDA）、乳酸脱氢酶（LDH）、肌酸激酶（CK）活性、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、糖原和细胞活力。在体内研究中，ICR雄性小鼠每日口服TA或蒸馏水28天。然后，在最后一天进行FST和TST。此外，还进行了血清、肌肉和肝脏的生化分析。TA剂量依赖地减轻了H2O2刺激的C2C12细胞中MDA、LDH、CK活性、TNF-α和IL-6的水平，而不影响细胞毒性。TA增加了H2O2刺激的C2C12细胞中SOD和CAT活性以及糖原水平。在TST和FST动物模型中，TA显著减少了FST的静止时间，同时增加了TST的持久时间，而没有影响体重波动。体内研究显示，TA的给药使SOD、CAT、柠檬酸合酶、糖原和游离脂肪酸水平升高，而显著降低了葡萄糖、MDA、LDH、乳酸、CK、炎症细胞因子、丙氨酸转氨酶、天门冬氨酸转氨酶、血尿素氮和皮质醇水平与对照组相比。TA通过调节C2C12细胞和动物模型中的氧化应激和能量代谢来改善疲劳。因此，我们建议TA可以成为改善疲劳的健康功能食品和治疗药物的有效物质。©2023 The Korean Nutrition Society and the Korean Society of Community Nutrition.

Oxidative stress is caused by reactive oxygen species and free radicals that accelerate inflammatory responses and exacerbate fatigue. Tormentic acid (TA) has antioxidant and anti-inflammatory properties. Thus, the aim of present study is to determine the fatigue-regulatory effects of TA in H2O2-stimulated myoblast cell line, C2C12 cells and treadmill stress test (TST) and forced swimming test (FST) animal models.In the in vitro study, C2C12 cells were pretreated with TA before stimulation with H2O2. Then, malondialdehyde (MDA), lactate dehydrogenase (LDH), creatine kinase (CK) activity, tumor necrosis factor (TNF)-α, interleukin (IL)-6, superoxide dismutase (SOD), catalase (CAT), glycogen, and cell viability were analyzed. In the in vivo study, the ICR male mice were administered TA or distilled water orally daily for 28 days. FST and TST were then performed on the last day. In addition, biochemical analysis of the serum, muscle, and liver was performed.TA dose-dependently alleviated the levels of MDA, LDH, CK activity, TNF-α, and IL-6 in H2O2-stimulated C2C12 cells without affecting the cytotoxicity. TA increased the SOD and CAT activities and the glycogen levels in H2O2-stimulated C2C12 cells. In TST and FST animal models, TA decreased the FST immobility time significantly while increasing the TST exhaustion time without weight fluctuations. The in vivo studies showed that the levels of SOD, CAT, citrate synthase, glycogen, and free fatty acid were increased by TA administration, whereas TA significantly reduced the levels of glucose, MDA, LDH, lactate, CK, inflammatory cytokines, alanine transaminase, aspartate transaminase, blood urea nitrogen, and cortisol compared to the control group.TA improves fatigue by modulating oxidative stress and energy metabolism in C2C12 cells and animal models. Therefore, we suggest that TA can be a powerful substance in healthy functional foods and therapeutics to improve fatigue.©2023 The Korean Nutrition Society and the Korean Society of Community Nutrition.