皮肤是人体最外层的器官，起到保护作用，抵御日光和污染等外部环境的损害，这些损害会影响抗氧化防御和皮肤炎症，导致红斑或皮肤发红，免疫抑制和表皮DNA损伤。本研究旨在调查红橙复合物H提取物（ROC）对Skh:HR-2小鼠的紫外线（UV）诱导的皮肤光老化的潜在保护作用。ROC以每日20、40和80 mg/kg的剂量经口给药，连续13周，并与小鼠进行为期10周的UV照射。ROC改善了UV诱导的皮肤屏障参数，包括红斑、黑色素生成、角质层水分损失、弹性和皱纹形成。值得注意的是，ROC抑制了炎症因子（白细胞介素6和肿瘤坏死因子α）和黑色素形成的mRNA表达。此外，通过增强基因表达，ROC还恢复了UV诱发的透明质酸和胶原蛋白水平的降低。此外，ROC显著下调了负责胶原蛋白降解的基质金属蛋白酶的蛋白和mRNA表达。ROC对光老化的保护作用与抑制UV诱导的c-Jun NH2末端激酶的磷酸化和旁路蛋白1激活有关。总之，我们的研究结果表明，ROC的口服给药具有潜在的对UV照射无毛小鼠的光老化的保护作用。©2023 The Korean Nutrition Society and the Korean Society of Community Nutrition.

The skin is the outermost organ of the human body and plays a protective role against external environmental damages, such as sunlight and pollution, which affect anti-oxidant defenses and skin inflammation, resulting in erythema or skin reddening, immunosuppression, and epidermal DNA damage.The present study aimed to investigate the potential protective effects of red orange complex H extract (ROC) against ultraviolet (UV)-induced skin photoaging in Skh:HR-2 mice. ROC was orally administered at doses of 20, 40, and 80 mg/kg/day for 13 weeks, along with UV irradiation of the mice for 10 weeks.ROC improved UV-induced skin barrier parameters, including erythema, melanin production, transepidermal water loss, elasticity, and wrinkle formation. Notably, ROC inhibited the mRNA expression of pro-inflammatory cytokines (interleukin 6 and tumor necrosis factor α) and melanogenesis. In addition, ROC recovered the UV-induced decrease in the hyaluronic acid and collagen levels by enhancing genes expression. Furthermore, ROC significantly downregulated the protein and mRNA expression of matrix metalloproteinases responsible for collagen degradation. These protective effects of ROC against photoaging are associated with the suppression of UV-induced phosphorylation of c-Jun NH2-terminal kinase and activator protein 1 activation.Altogether, our findings suggest that the oral administration of ROC exerts potential protective activities against photoaging in UV-irradiated hairless mice.©2023 The Korean Nutrition Society and the Korean Society of Community Nutrition.