手持式离子敏感场效应晶体管实验室芯片检测TMPRSS2-ERG和AR mRNA在前列腺癌预后中的应用。
Handheld ISFET Lab-on-Chip detection of TMPRSS2-ERG and AR mRNA for prostate cancer prognostics.
发表日期：2023 Aug
作者：
Joseph Broomfield, Melpomeni Kalofonou, Sylvia Franklin, Sue M Powell, Thomas Pataillot-Meakin, Nicolas Moser, Charlotte L Bevan, Pantelis Georgiou
来源：
BIOSENSORS & BIOELECTRONICS
摘要：
离子敏感场效应晶体管 ( ISFETs ) 结合未经改造的互补金属氧化物半导体 (CMOS) 是临床诊断和预后的现场平台。该研究证明了对前列腺癌两个循环 mRNA 标记物即雄激素受体和 TMPRSS2-ERG 融合基因的敏感和特异性检测，采用了目标特异性的环介导等温扩增方法。TMPRSS2-ERG 和雄激素受体 RNA 可以在30分钟内检测到 3x101 和 5x101份拷贝。这些试剂应用在ISFET Lab-on-chip器件上成功，且通过前列腺癌细胞系提取的 mRNA 证实了每个标记物的特异性。
Ion-sensitive field-effect transistors (ISFETs) in combination with unmodified complementary metal oxide semiconductors present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive and specific detection of two circulating mRNA markers for prostate cancer, the androgen receptor and the TMPRSS2-ERG fusion using a target-specific loop-mediated isothermal amplification method. TMPRSS2-ERG and androgen receptor RNA were detected down to 3x101 and 5x101 copies respectively in under 30 minutes. Administration of these assays onto the ISFET Lab-on-chip device was successful and the specificity of each marker was corroborated with mRNA extracted from prostate cancer cell lines.