使用哺乳动物细胞评估了植物提取物对Caspase 3、Caspase 8和BID基因在死亡受体诱导途径下的表达谱的抑制增殖作用。本研究检测了姜黄（Curcuma longa）和印度醉鱼果（Emblica officinalis）这两种药用植物提取物对Vero和MDA-MB 231细胞株的抑制增殖作用，并通过中性红染料摄取实验进行评估。使用逆转录聚合酶链反应确定基因的表达，其中GAPDH表达作为内部对照。在经甲醇姜黄提取物诱导的MDA-MB 231细胞中，BID的表达上调，而Caspase 3和Caspase 8的表达在诱导和未诱导的MDA-MB 231细胞中相同。活化的BID被裂解成tBID，并激活内在途径，导致甲醇姜黄提取物诱导的癌细胞死亡。姜黄乙醇提取物对Vero细胞和甲醇提取物对MDA-MB 231细胞表现出最强的抑制增殖作用。细胞系的形态学研究和姜黄甲醇提取物处理细胞的基因表达分析显示，通过将BID转化为t-BID（内在途径），激活Caspase-3和Caspase-8（外在途径），姜黄甲醇提取物引发了凋亡的活化。通过与未诱导的癌细胞相比，在诱导的癌细胞中观察到不同的细胞毒性和凋亡诱导，因此姜黄是天然的新型抗癌化合物的来源。

Plant extracts antiproliferative effects were determined by using mammalian cells along the expression profile of Caspases 3, 8 and the BID gene of the death receptor-induced pathway. Two medicinal plants viz., Turmeric (Curcuma longa) and Amla (Emblica officinalis) extracts were examined for antiproliferative effect through Neutral Red-Dye uptake assay on Vero and MDA-MB 231 cell lines. A reverse transcriptase polymerase chain reaction was used to determine the expression of genes while GAPDH expression was used as an internal control. Expression of BID was up-regulated in methanolic turmeric extract-induced MDA-MB 231 cells while Caspases 3,8 expressions were the same in induced and uninduced MDA-MB 231 cells. Activated BID cleaved into tBID and activated the intrinsic pathway which caused death in methanolic turmeric extract-induced cancerous cells. Ethanolic extracts of turmeric exerted the strongest antiproliferative effects on Vero and methanolic extracts on MDA-MB 231 cells. The morphological studies of cell lines and gene expression analysis of turmeric methanolic extract-treated cells showed activation of apoptosis via converting BID into t-BID (intrinsic pathway) and activating Caspase-3 and Caspase-8 (extrinsic pathway). With the differential cytotoxicity and induction of apoptosis in induced cancer cells in comparison to uninduced cancerous cells, hence turmeric is a natural source of new anti-cancerous compounds.