使用整个肺部图谱在单细胞水平上进行纳米粒子和肿瘤的跨尺度追踪。
Cross-scale tracing of nanoparticles and tumors at the single-cell level using the whole-lung atlas.
发表日期：2023 Aug 02
作者：
Zeying Cao, Yanli Zhao, Hongyu Sun, Xian Sun, Yu Zhang, Shuo Zhang, Caifen Wang, Ting Xiong, Abid Naeem, Jiwen Zhang, Xianzhen Yin
来源：
Food & Function
摘要：
目前，肿瘤异质性对于肿瘤治疗的有效性构成了重大挑战，这也对纳米靶向药物传递系统（DDSs）的研发提出了巨大需求。因此，解决肿瘤与纳米颗粒之间的时空相互作用至关重要。然而，由于整个器官尺度与亚细胞精度之间存在差距，靶向评估受到颗粒可视化的限制。本研究提出了一种基于微观光学切片断层成像（MOST）系统和荧光MOST（fMOST）系统的高精度三维（3D）肿瘤结构可视化方法，以清晰地揭示肿瘤内纳米颗粒的3D空间分布情况。我们证明，通过MOST/fMOST系统，可以展现肿瘤结构和纳米颗粒分布之间的多维度和跨尺度相关性，从而重构肿瘤微环境并探索血管结构参数。与现有方法相比，这种可视化方法可以更准确地评估DDSs用于肿瘤治疗的功效、分布和靶向效率。
Currently, the effectiveness of oncotherapy is limited by tumor heterogeneities, which presents a huge challenge for the development of nanotargeted drug delivery systems (DDSs). Therefore, it is important to resolve the spatiotemporal interactions between tumors and nanoparticles. However, targeting evaluation has been limited by particle visualization due to the gap between whole-organ scale and subcellular precision. Here, a high-precision three-dimensional (3D) visualization of tumor structure based on the micro-optical sectioning tomography (MOST) system and fluorescence MOST (fMOST) system is presented to clarify 3D spatial distribution of nanoparticles within the tumor. We demonstrate that through the MOST/fMOST system, it is possible to reveal multidimensional and cross-scale correlations between the tumor structure and nanoparticle distribution to remodel the tumor microenvironment and explore the structural parameters of vasculature. This visualization methodology provides an accurate assessment of the efficacy, distribution, and targeting efficiency of DDSs for oncotherapy compared to available approaches.