通过激活线粒体介导的凋亡途径，转-茴香醚在人肝癌细胞系HepG2细胞中诱导凋亡。
Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways.
发表日期：2023 Jul 31
作者：
Steve Harakeh, Rajaa Al-Raddadi, Turki Alamri, Soad Al-Jaouni, Mohammed Qari, Yousef Qari, Ajay Kumar, Hanaa M Tashkandi, Mohammed Moulay, Alia M Aldahlawi, Petr Slama, Shafiul Haque
来源：
Stem Cell Research & Therapy
摘要：
茴香酮是从八角果中提取的一种有价值的化合物，被广泛用于民族部落治疗多种人类疾病。本研究探讨了茴香酮对人肝癌细胞（HepG2）、宫颈癌细胞（HeLa）和乳腺癌细胞（MCF-7）的抗增殖活性。DNA切割实验显示，茴香酮具有自由基清除潜力。与其他细胞系相比，茴香酮对HepG2细胞表现出较强的抗增殖潜力。Western blotting分析显示，茴香酮显著上调了p53、Caspase-3和Caspase-9的蛋白表达水平，这表明茴香酮对HepG2细胞有诱导凋亡的能力。Rt-qPCR分析显示，与未经处理的HepG2癌细胞相比，茴香酮上调了p53、caspase-3和caspase-9的表达水平。此外，茴香酮引起了ROS的生成和线粒体膜电位的破坏。我们的研究表明，茴香酮可能具有显著的抗癌治疗潜力，可用于治疗肝癌。版权所有 © 2023 作者。由Elsevier Masson SAS出版。保留所有权利。
trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase - 3 and - 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.