癌症诊断近年来一直是医学研究的前沿，持续努力开发用于检测患者癌症的设备和技术。癌症诊断的一个有希望的方法是在血样中检测循环肿瘤细胞（CTCs）。将这些罕见的细胞从多样的血细胞中分离并分析它们可以提供对疾病的分期和致死性的宝贵见解。在这里，我们介绍了一种在聚合物盘上利用离心力进行细胞分离的离心微流控平台的设计和制作。分离单元利用了主动和被动方法。换句话说，除了引入新的通道几何形状，还使用外部磁场将靶细胞与背景细胞分离。为了使外部场起作用，CTCs必须先用与抗体共轭的纳米颗粒标记，然后进行分离。在实验测试之前，进行了数值研究以确定最佳参数；角速度和磁化研究显示2000 rpm和868,000（kA/m）是设计设备达到白细胞（WBCs）和CTCs 100％效率的最佳条件。这些结果表明，通道的被动区域主要有助于目标细胞的聚焦，并且显示了扩张-收缩几何形状与锯齿几何形状相比，聚焦效果更明显。此外，结果证明，弯曲的通道几何形状在分离效率方面比直线几何形状表现更好。然而，如果分离仅依靠通道几何形状，大多数细胞会被引导到非靶向腔室，导致结果不理想。这是由于作用在细胞上的力的方向。然而，包括外部磁场可以改善净力的方向并提高分离效率。最后，将数值和实验结果进行了比较，并将曲线膨胀-收缩通道引入为具有100％和约92％ CTC 分离效率的最佳几何形状。版权所有©2023 Elsevier B.V. 保留所有权利。

Cancer diagnosis has recently been at the forefront of recent medical research, with ongoing efforts to develop devices and technologies for detecting cancer in patients. One promising approach for cancer diagnosis is the detection of Circulating Tumor Cells (CTCs) in blood samples. Separating these rare cells from the diverse background of blood cells and analyzing them can provide valuable insights into the disease's stage and lethality. Here we present the design and fabrication of a centrifugal microfluidic platform on a polymeric disk that utilizes centrifugal forces for cell isolation. The separation units exploit both active and passive methods. In other words, in addition to introducing novel geometry for channels, an external magnetic field is also employed to separate the target cells from the background cells. In order for the external field to function, the CTCs must first be labeled with antibody-conjugated nanoparticles; the separation process should be then performed. Before the experimental tests, a numerical study was done to determine the optimum parameters; the angular velocity and magnetization investigations showed that 2000 rpm and 868,000 (kA/m) are the optimum conditions for the designed device to reach the efficiency of 100% for both White Blood Cells (WBCs) and CTCs. These results indicate that the passive region of the channels primarily contributes to the focusing of the target cells, and showed that the focusing effect is more pronounced in the expansion-contraction geometry compared to the zigzag geometry. Additionally, the results proved that curved channel geometries performed better than straight ones in terms of separation efficiency. However, if the separation relies solely on channel geometry, the majority of cells would be directed towards the non-target chamber, leading to suboptimal results. This is due to the direction of the forces acting on the cells. However, including an external magnetic field improves the direction of the net force and enhances the separation efficiency. Finally, the numerical and experimental results of the study were compared, and the curved expansion-contraction channel is introduced as the best geometry having 100% and ∼92% CTC separation efficiency, respectively.Copyright © 2023 Elsevier B.V. All rights reserved.