纳米多孔硅化物现已广泛应用于纳米和微米材料研究的各个领域。纳米多孔材料的优势在于它可以被各种亲水和疏水性分子填充，并将其传递至目标细胞和组织。在本研究中，我们研究了纳米多孔硅化物与疏水和光动力活性分子——叶克蓝的相互作用。叶克蓝被吸附在/内SBA-15纳米多孔硅化物中，导致其荧光消失，由于叶克蓝形成聚集体。然而，我们观察到叶克蓝可以轻易地从这些颗粒中重新分配至体外和体内的血清和细胞中的蛋白质和脂质。此外，SBA-15孔道的带电表面特性迫使颗粒表面形成蛋白质/脂质包被。这种复杂结构使得叶克蓝在包被中以单体形式呈现出具有荧光的光环并产生适用于光动力治疗(PDT)的单线态氧。通过将新构建引入PDT方案中，所实现的PDT疗效与叶克蓝PDT的疗效相当。总之，本研究证明了使用纳米多孔硅化物通过荧光技术向癌细胞输送疏水光敏剂的一种有前景的方法。©2023 Elsevier B.V. All rights reserved.

Nanoporous silica is nowadays used in various fields of nano- and micro-materials research. The advantage of nanoporous material is that it can be filled with various hydrophilic and hydrophobic molecules, which are then delivered to the target cells and tissues. In the present study, we have studied the interaction of nanoporous silica with hydrophobic and photodynamically active molecule - hypericin. Hypericin was adsorbed on/in SBA-15 silica, which led to the disappearance of its fluorescence due to hypericin aggregate formation. However, it was observed here that hypericin can be easily redistributed from these particles towards proteins and lipids in serum and cells in vitro and in vivo. Moreover, the charged surface character of SBA-15 pores forced the creation of protein/lipid corona on particles. Such complex enabled monomerization of hypericin on the surface of particles presented by fluorescence in the corona and singlet oxygen production suitable for photodynamic therapy (PDT). The PDT efficacy achieved by introducing the new construct into the PDT protocol was comparable to the efficacy of hypericin PDT. In conclusion, this study demonstrates a promising approach for the delivery of hydrophobic photosensitizers to cancer cells by nanoporous silica using fluorescence techniques.Copyright © 2023 Elsevier B.V. All rights reserved.