该研究调查了亚麻籽中的亚麻杆二糖苷（SDG）提取物对实验性肥胖中凋亡、蛋白质刺刺（Hh）、自噬和抗氧化过程的影响。40只大鼠被分为两组，分别接受常规平衡饮食或高脂饮食，并分成四个组：GI：对照组在12周内接受常规饮食。GII：动物接受高脂饮食和盐水胃灌注。GIII：HFD肥胖大鼠经口给予SDG提取物（10 mg/kg/b.w.）和含有1.18 mg SDG/kg的饲料治疗4周。GIV：同时接受正常平衡饮食和SDG提取物（10 mg/kg/b.w.）以及含有1.18 mg SDG/kg的饲料治疗12周的正常平衡饮食大鼠。SDG提取物的给予导致体重、葡萄糖、血脂和瘦素在肥胖组中显著下降。它还改善了抗氧化水平（降低了丙二醛水平，同时增加了总抗氧化能力）和抗炎状态（降低了白细胞介素-6和肿瘤坏死因子α的水平）。SDG提取物通过调节自噬基因和凋亡蛋白，降低了Hh基因的表达水平（包括蛋白质裂解同源物1、Hh相互作用蛋白、胶质瘤相关癌基因同源物1和光滑蛋白受体）。亚麻杆二糖苷提取物改善了肥胖大鼠的抗炎和抗氧化状态，并通过调节Hh基因、自噬基因和凋亡蛋白的表达水平，暗示其可以通过调节氧化、炎症、自噬和凋亡来预防和管理肥胖及其相关并发症。对SDG的自噬途径进行进一步研究，以解决肥胖及其并发症问题，是必须的。版权声明：©Journal of Advanced Veterinary and Animal Research.

This research investigated secoisolariciresinol diglucoside (SDG) flax extract effects on apoptosis, hedgehog (Hh), autophagy, and the anti-oxidation process in experimentally induced obesity.Forty rats were separated into two sets regarding either receiving a normal balanced diet or a high-fat diet (HFD) and then distributed into four groups: GI: The control group had a regular diet for 12 weeks. GII: animals received a high-fat meal and saline by gastric gavage. GIII: HFD obese rats treated with SDG extract orally (10 mg/kg/b.w.) and 1.18 mg SDG/kg in the diet for 4 weeks GIV: Normal balanced diet rats received SDG extract orally (10 mg/kg/b.w.) and 1.18 mg SDG/kg of chow for 12 weeks in addition to their regular balanced diet.The administration of SDG extract exhibited a significant drop in body weight, glucose, lipid profile, and leptin compared to the obese group. It also improved the antioxidant levels (lowering the levels of malondialdehyde while increasing the total antioxidant capacity) and anti-inflammatory status (decreasing interleukin-6 and tumor necrosis factor-alpha). SDG extract downregulates the expression of HH genes (protein patched homolog 1, Hh-interacting protein, glioma-associated oncogene homolog 1, and smoothened receptor) in conjunction with the modulation of autophagy genes and apoptotic proteins.SDG extract showed improved anti-inflammatory and antioxidant status and downregulated the expression of HH genes while modulating autophagy genes and apoptotic proteins among obese rats, suggesting that it may be used to avert and manage obesity and its correlated complications by modulating oxidation, inflammation, autophagy, and apoptosis. Advanced future research on the SDG autophagy pathway to address obesity and its complications is mandatory.Copyright: © Journal of Advanced Veterinary and Animal Research.