对一个患有骨髓性白血病的老年患者进行单细胞测序结果分析发现，在单核细胞和造血干细胞中存在多个癌基因的高表达。
Analysis of single-cell sequencing results of an elderly patient with myeloid leukemia reveals high expression of multiple oncogenes in monocytes and hematopoietic stem cells.
发表日期：2023 Dec
作者：
Xiaoli Xu, Minjian Xiong, Haiyan Ye, Yonglei Qi, Ying Zhao
来源：
Cell Death & Disease
摘要：
年龄较大的急性髓系白血病（AML）患者面临更高的死亡风险。本研究对一位64岁的AML-M5患者进行了基因测序结果的分析，旨在为治疗提供新的思路。本研究对该患者在化疗前的外周血和骨髓中的血细胞进行了单细胞RNA测序（scRNA-seq）生物信息学分析，鉴定出差异表达基因（DEGs），并进行了功能富集分析。在单核细胞和造血干细胞（HSCs）中共鉴定出7990和123个差异表达基因（DEGs）。在前40个分析的DEGs中，MYB在外周血单核细胞中表达较高，而其他13个与肿瘤相关的基因在骨髓单核细胞中表达较高。外周血和骨髓中的造血干细胞分别有6和12个高度表达的与肿瘤相关的基因，包括MCL1、JUN和JUNB。这些基因可能形成一个相互连接的网络，对AML的进展和异质性有影响，对患者的治疗和预后具有影响。总之，在治疗年长的AML患者时，考虑其个体特征以优化治疗策略非常重要。
Older patients with acute myeloid leukemia (AML) face a higher risk of death. This study analyzed the gene sequencing results of an elderly AML patient to provide new ideas for treatment.This study performed single-cell RNA sequencing (scRNA-seq) bioinformatics analysis of blood cells in the peripheral blood and bone marrow of a 64-year-old AML-M5 patient before chemotherapy. The differentially expressed genes (DEGs) were identified, and functional enrichment analyses were performed.A total of 7990 and 123 DEGs were identified in monocytes and hematopoietic stem cells (HSCs), respectively. Among the top 40 DEGs analyzed, MYB showed high expression in peripheral blood monocytes, while 13 other tumor-related genes exhibited high expression in monocytes in the bone marrow. Peripheral blood and bone marrow HSCs had 6 and 12 highly expressed tumor-related genes respectively, including MCL1, JUN, and JUNB. These genes may form a interconnected network contributing to the progression and heterogeneity of AML, which can have an impact on patient treatment and prognosis.In conclusion, when treating elderly AML patients, it is important to consider their individual characteristics in order to optimize treatment strategies.