CDC20通过激活Twist1表达促进前列腺癌的放射线耐药性。
CDC20 promotes radioresistance of prostate cancer by activating Twist1 expression.
发表日期：2023 Aug 03
作者：
Chuanlai Yang, Yuegang Ge, Yachen Zang, Ming Xu, Lu Jin, Yang Wang, Xinyu Xu, Boxin Xue, Zhiwei Wang, Lixia Wang
来源：
Cellular & Molecular Immunology
摘要：
目前，放射治疗是前列腺癌（PCa）患者最具吸引力的治疗之一。然而，放射抗性仍然是一个具有挑战性的问题，其潜在机制尚不清楚。越来越多的证据显示，细胞周期蛋白20（CDC20）在多种肿瘤中起着关键作用，包括PCa。在这里，通过对GEPIA数据库的挖掘以及西方印迹分析，我们发现CDC20在PCa组织和细胞中表达较高。我们证明了辐射能够增加PCa细胞中CDC20的表达，而CDC20的沉默则抑制了细胞增殖、迁移、肿瘤形成，诱导了细胞凋亡，并提高了PCa体外和体内的放射敏感性。此外，我们观察到CDC20通过调控Twist1通路影响细胞增殖和迁移。这些结果表明，靶向CDC20和Twist1可能是改善PCa放射敏感性的有效途径。© 2023. 该作者授予Springer Science+Business Media, LLC的独家许可，属于Springer Nature的一部分。
Currently, radiotherapy is one of the most attractive treatments for prostate cancer (PCa) patients. However, radioresistance remains a challenging issue and the underlying mechanism is unknown. Growing evidence has demonstrated that CDC20 (Cell division cycle protein 20) plays a pivotal role in a variety of tumors, including PCa. Here, GEPIA database mining and western blot analysis showed that higher expression of CDC20 was observed in PCa tissues and cells. We demonstrated that the expression of CDC20 was increased in PCa cells by irradiation, and knockdown of CDC20 resulted in inhibition of cell proliferation, migration, tumor formation, induced cell apoptosis and increased radiosensitivity in PCa in vitro and in vivo. Furthermore, we observed that CDC20 regulated Twist1 pathway, influencing cell proliferation and migration. These results suggest that targeting CDC20 and Twist1 may be an effective way to improve the radiosensitivity of PCa.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.