建立和表征源自中国高钙型小细胞卵巢癌患者的一个新的细胞系(SCCOHT-CH-1)和人源异种移植模型。
Establishment and characterization of a novel cell line (SCCOHT-CH-1) and PDX models derived from Chinese patients of small cell ovarian carcinoma of the hypercalcemic type.
发表日期:2023 Aug 03
作者:
Yi Gao, Kewei Zheng, Mingyi Kang, Jing Xu, Yan Ning, Weiguo Hu, Ke Li, Yu Kang, Congjian Xu
来源:
Cellular & Molecular Immunology
摘要:
卵巢高钙型小细胞癌(SCCOHT)是一种罕见且具侵袭性的恶性肿瘤,由于其恶性预后,对临床提出了重大挑战。不幸的是,目前仅有三种SCCOHT细胞系可供科学研究使用。在本研究中,我们成功建立了一种新型SCCOHT细胞系,命名为SCCOHT-CH-1,该细胞系来源于SCCOHT患者的复发病变。我们采用形态观察、CCK-8法、Transwell法、克隆形成法、短串联重复序列(STR)分析、核型分析、免疫组织化学染色法、免疫印迹法和异种移植瘤形成实验等技术对这种新型细胞系进行了全面的表征。SCCOHT-CH-1细胞呈小圆形,具有独特的STR谱。SCCOHT-CH-1的人群倍增时间为33.02小时。该细胞系显示出潜在的迁移和侵袭能力。与另一种SCCOHT细胞系COV434相比,SCCOHT-CH-1表现出更高的AKT、VIM和CCND1表达。同时,SCCOHT-CH-1具有在体内形成肿瘤的能力。我们还成功构建了三种SCCOHT的患者源异种移植瘤(PDX)模型,病理诊断与原发肿瘤一致,并伴有SAMRCA4蛋白表达的丧失。从中国人中建立SCCOHT-CH-1细胞系和PDX模型代表了揭示SCCOHT分子机制和发展针对这种具有挑战性恶性肿瘤的靶向干预的关键步骤。© 2023. 作者。
Small cell carcinoma of the ovary hypercalcemic type (SCCOHT) is a rare and aggressive malignancy that poses a significant clinical challenge due to its grim prognosis. Unfortunately, only three SCCOHT cell lines are currently available for scientific research. In this study, we have successfully established a novel SCCOHT cell line from a recurrent lesion of a SCCOHT patient, named SCCOHT-CH-1. We comprehensively characterized the novel cell line by employing techniques such as morphological observation, CCK-8 assay, Transwell assay, clone formation assay, short tandem repeat sequence (STR) analysis, karyotype analysis, immunohistochemical staining, western blot assay, and xenograft tumor formation assay. SCCOHT-CH-1 cells were small circular and had a unique STR profile. The population-doubling time of SCCOHT-CH-1 was 33.02 h. The cell line showed potential migratory and invasive ability. Compared with another SCCOHT cell line COV434, SCCOHT-CH-1 exhibited higher expression of AKT, VIM, and CCND1. At the same time, SCCOHT-CH-1 has the ability of tumorigenesis in vivo. We also successfully constructed three patient-derived xenograft (PDX) models of SCCOHT, which were pathologically diagnosed to be consistent with the primary tumor, accompanied by loss of SAMRCA4 protein expression. The establishment of SCCOHT-CH-1 cell line and PDX models from Chinese people represent a pivotal step toward unraveling the molecular mechanism of SCCOHT and fostering the development of targeted interventions to tackle this challenging malignancy.© 2023. The Author(s).