人类肠道器官样本作为研究肠道细菌和病毒的模型。
Human intestinal organoids as models to study enteric bacteria and viruses.
发表日期：2023 Aug 01
作者：
Grace Adeniyi-Ipadeola, Hephzibah Nwanosike, Sasirekha Ramani
来源：
CURRENT OPINION IN MICROBIOLOGY
摘要：
历史上，通过使用转化细胞系和动物模型对宿主-微生物相互作用进行了实验室研究。虽然从这些模型中学到了很多东西，但最近在多细胞、生理活性的人类肠道器官样体（HIO）培养的发展方面取得的突破性进展使得对宿主-微生物相互作用的发现前所未有。在这里，我们对使用HIO作为模型研究临床重要肠道细菌和病毒的致病机制以及研究共生肠道微生物的最新文献进行综述。我们还讨论了当前HIO培养系统的局限性以及技术进步和创新工程方法如何提供改善模型的新方向。这些研究的讨论突出了HIO在研究微生物致病机制、宿主-微生物相互作用以及临床前治疗和疫苗开发方面的潜力。版权所有 © 2023 Elsevier Ltd. 保留所有权利。
Laboratory studies of host-microbe interactions have historically been carried out using transformed cell lines and animal models. Although much has been learned from these models, recent advances in the development of multicellular, physiologically active, human intestinal organoid (HIO) cultures are allowing unprecedented discoveries of host-microbe interactions. Here, we review recent literature using HIOs as models to investigate the pathogenesis of clinically important enteric bacteria and viruses and study commensal intestinal microbes. We also discuss limitations of current HIO culture systems and how technical advances and innovative engineering approaches are providing new directions to improve the model. The studies discussed here highlight the potential of HIOs for studying microbial pathogenesis, host-microbe interactions, and for preclinical development of therapeutics and vaccines.Copyright © 2023 Elsevier Ltd. All rights reserved.