黄花蒿（Artemisia anomala S. Moore）具有多种药理活性，包括解除血瘀、退热镇痛、消肿除湿。本研究对从黄花蒿中提取的总黄酮类化合物进行了提取工艺、化学成分及其体外抗炎作用和机制的研究。通过单因素实验和响应面法优化了总黄酮类提取物的最佳提取率，并采用UPLC-QTOF-MS方法分析了化学成分；利用脂多糖诱导的RAW 264.7细胞评估了提取物的抗炎活性。在乙醇浓度为50％、超声提取时间为30分钟、溶剂体积与物料重量之比为20：1（ml/g）的条件下，最高提取率为2.02％。此外，通过质谱信息和相关文献，初步鉴定了总黄酮提取物的主要成分，并证明其通过减少吞噬作用、一氧化氮含量和相关细胞因子（肿瘤坏死因子-α，白细胞介素-10，白细胞介素-6）的水平来展示出较强的抗炎活性。此外，进一步揭示了提取物的抗炎作用与TLR4-MyD88-NF-κB信号通路的激活密切相关。该研究表明，黄花蒿总黄酮提取物可能是一种更好的候选抗炎天然药物。版权所有©2023。Elsevier B.V.出版。

Artemisia anomala S. Moore exerts many pharmacological activities, including the removing of the blood stasis, relieving of the fever and analgesia, reducing the swelling and dampness. In this study, the extraction technology, chemical compositions and anti-inflammatory effect in vitro and mechanism of total flavonoids extract from Artemisia anomala S. Moore were studied. The optimal yield rate of total flavonoids extract was optimized by single factor experiments and response surface method, and the chemical constituents were analyzed by UPLC-QTOF-MS method; and the anti-inflammatory activity of the extract was evaluated with lipopolysaccharide induced RAW 264.7 cells. The highest extraction rate was 2.02% under these conditions of the concentration of ethanol 50%, the ultrasonic extraction time 30 min, and the ratio of solvent volume to material weight 20:1 (ml/g). In addition, the main components of total flavonoid extract were preliminarily identified and deduced based on mass spectrometry information and relevant literatures, and its stronger anti-inflammatory activity was demonstrated by reducing the phagocytosis, the content of nitric oxide and the level of related cytokines (tumor necrosis factor-α, interleukin-10, interleukin-6). Furthermore, it was further revealed that the anti-inflammatory effect of the extract was closely connected with the activation of TLR4-MyD88-NF-κB signalling pathway. This study indicated that the total flavonoids extract from Artemisia anomala S. Moore may be a better candidate anti-inflammatory natural medicine.Copyright © 2023. Published by Elsevier B.V.