烹饪油烟（COFs）由一系列致癌的挥发性有机醛类化合物（VOAs）组成，特别是反式-反式-2,4-癸二醛（t,t-DDE）、4-羟基己醛（4-HHE）和4-羟基壬醛（4-HNE）。监测不同烹饪条件下人体暴露于有毒VOAs的水平对于预测癌症风险至关重要。为此，我们开发了一种快速组织提取（FaTEx）技术与超高效液相色谱串联质谱（UHPLC-MS/MS）相结合，以监测小鼠肺组织样品中的三种有毒VOAs。FaTEx预处理方案通过结合两个注射器进行提取和清洗过程而开发。优化了影响FaTEx样品预处理过程的各个步骤，以增强样品基质中目标VOAs的提取效率。在最佳实验条件下，结果显示良好的相关系数值>0.99，检测限在0.5-3 ng/g之间，定量限在1-10 ng/g之间，基质效应<18.1%。此外，加入样品的提取回收率在88.9-109.6%之间，相对标准偏差（RSD）<8.6%。对不同时间段接触含有t,t-DDE的烹饪油烟的小鼠进行了牺牲以验证所开发的技术，结果显示t,t-DDE浓度在14.8至33.8 μg/g之间。所得结果显示这是一种快速、可靠且半自动化的样品预处理技术，具有良好的提取效率、痕量检测限和较小的基质效应。因此，该方法可作为一种潜在的分析方法，用于测定长期暴露于烹饪油烟的人体中的VOAs。版权所有 © 2023 Elsevier Ltd. 出版。

Cooking oil fumes (COFs) comprised of a mixture of cancer-causing volatile organic aldehydes (VOAs), particularly trans, trans-2,4-decadienal (t,t-DDE), 4-hydroxy-hexenal (4-HHE), and 4-hydroxy-nonenal (4-HNE). Monitoring toxic VOAs levels in people exposed to different cooking conditions is vital to predicting the cancer risk. For this purpose, we developed a fast tissue extraction (FaTEx) technique combined with UHPLC-MS/MS to monitor three toxic VOAs in mice lung tissue samples. FaTEx pre-treatment protocol was developed by combining two syringes for extraction and clean-up process. The various procedural steps affecting the FaTEx sample pre-treatment process were optimized to enhance the target VOAs' extraction efficiency from the sample matrix. Under the optimal experimental conditions, results exhibit good correlation coefficient values > 0.99, detection limits were between 0.5-3 ng/g, quantification limits were between 1-10 ng/g, and the matrix effect was <18.1%. Furthermore, the extraction recovery values of the spiked tissue exhibited between 88.9-109.6% with <8.6% of RSD. Cooking oil fume (containing t,t-DDE) treated mice at various time durations were sacrificed to validate the developed technique, and it was found that t,t-DDE concentrations were from 14.8 to 33.8 μg/g. The obtained results were found to be a fast, reliable, and semi-automated sample pre-treatment technique with good extraction efficiency, trace level detection limit, and less matrix effect. Therefore, this method can be applied as a potential analytical method to determine the VOAs in humans exposed to long-term cooking oil fumes.Copyright © 2023. Published by Elsevier Ltd.