TCF-1（T细胞因子-1）阳性干细胞样肿瘤浸润淋巴细胞（干细胞样TILs）是肿瘤微环境中重要的记忆细胞。然而，它们与肺腺癌（LUAD）的临床病理特征，CD8+ TIL密度，免疫检查点抑制剂（ICs）和预后价值的关系尚不清楚。本研究旨在表征TCF-1+ TILs及其在接受手术切除的LUAD患者中的预后意义。利用免疫组织化学对切除的LUAD进行了TCF-1、CD8和PD-1、LAG-3以及TIM-3等ICs在TILs中的表达量估计。分析TCF-1表达与患者预后的临床病理特征之间的关联。TCF-1阳性表达与晚期病理分期、肿瘤分级、CD8+ TIL密度、TIM-3表达、LAG-3表达和PD-1表达显著相关。TCF-1阳性与更好的无复发生存期（RFS）和总生存期（OS）显著相关。亚组分析显示，TCF-1+/CD8+组具有最佳的RFS和OS，而TCF-1-/CD8-组具有最差的RFS和OS。类似地，TCF-1 + PD-1-患者的预后最好，TCF-1-PD-1+患者的预后最差。在LUAD患者中，TCF-1在表达方面相对较高，具有特殊的临床病理特征。TCF-1+ TILs与CD8密度、TIM-3表达、LAG-3表达和PD-1表达相关，并与LUAD患者的更好预后相关。TCF-1和CD8密度或PD-1表达的组合可进一步将患者分层为具有不同预后的不同组。© 2023 作者.《胸腔癌》由中国肺癌研究组和John Wiley & Sons Australia, Ltd出版。

T cell factor-1 (TCF-1) + stem-like tumor-infiltrating lymphocytes (stem-like TILs) are important memory cells in the tumor microenvironment. However, their relationship with clinicopathological features, CD8+ TIL densities, immune checkpoint inhibitors (ICs), and prognostic values remain unknown for lung adenocarcinomas (LUADs). In this study, we aimed to characterize TCF-1+ TILs and their prognostic significance in patients with surgically resected LUADs.Expression of TCF-1, CD8, and ICs including programmed death-1 (PD-1), lymphocyte activating-3 (LAG-3), and T cell immunoglobulin and mucin-domain containing-3 (TIM-3) in TILs were estimated using immunohistochemistry of resected LUADs. The association between TCF-1 expressions and clinicopathological characteristics of patient prognoses were analyzed.Positive TCF-1 expression significantly correlated with advanced pathological stage, tumor grade, CD8+ TILs density, TIM-3 expression, LAG-3 expression, and PD-1 expression. TCF-1 positivity was significantly associated with a better recurrence-free survival (RFS), and overall survival (OS). Subgroup analysis revealed that the TCF-1+/CD8+ group had the best RFS and OS, while the TCF-1-/CD8- group had the worst RFS and OS. Similarly, patients with TCF-1 + PD-1- had the best prognoses and patients with TCF-1-PD-1+ had the worst prognoses.TCF-1 had relatively high positive expression and special clinicopathological features in patients with LUAD. TCF-1+ TILs were related to CD8 density, TIM-3 expression, LAG-3 expression, and PD-1 expression, and were associated with better prognoses in LUAD patients. A combination of TCF-1 and CD8 densities or PD-1 expression further stratified patients into different groups with distinct prognoses.© 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.