设计一种多功能且可回收的细胞宏包埋装置，用于治疗蛋白质的输送。
Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins.
发表日期：2023 Aug 18
作者：
Julien Grogg, Remi Vernet, Emily Charrier, Muriel Urwyler, Olivier Von Rohr, Valentin Saingier, Fabien Courtout, Aurelien Lathuiliere, Nicolas Gaudenzio, Adrien Engel, Nicolas Mach
来源：
CLINICAL PHARMACOLOGY & THERAPEUTICS
摘要：
微囊化细胞疗法在传递高效疗效的治疗蛋白至患者体内，改善慢性疾病管理方面具有巨大潜力。然而，目前尚未验证一种适用于临床应用的生物相容性、可扩展性和易于施用、取出或更换的多功能包埋装置。在这里，我们报告了一种适用于蛋白质传递的不受载体限制的大胶囊包埋装置，并优化了其功能特性。该装置与粘附和悬浮细胞兼容，并且可以在不繁琐的手术过程中施用和取出。我们表征了其生物相容性，并展示了不同细胞系产生的不同治疗蛋白质可以组合在该装置中。我们证明了植入到人体皮肤中的细胞因子分泌细胞在激活抗原呈递细胞方面的能力，这可能成为癌症免疫治疗中有效的佐剂策略。我们相信，我们的装置可能为癌症、代谢性疾病和蛋白质缺乏疾病的细胞疗法做出贡献。© 2023 作者
Encapsulated cell therapy holds a great potential to deliver sustained levels of highly potent therapeutic proteins to patients and improve chronic disease management. A versatile encapsulation device that is biocompatible, scalable, and easy to administer, retrieve, or replace has yet to be validated for clinical applications. Here, we report on a cargo-agnostic, macroencapsulation device with optimized features for protein delivery. It is compatible with adherent and suspension cells, and can be administered and retrieved without burdensome surgical procedures. We characterized its biocompatibility and showed that different cell lines producing different therapeutic proteins can be combined in the device. We demonstrated the ability of cytokine-secreting cells encapsulated in our device and implanted in human skin to mobilize and activate antigen-presenting cells, which could potentially serve as an effective adjuvant strategy in cancer immunization therapies. We believe that our device may contribute to cell therapies for cancer, metabolic disorders, and protein-deficient diseases.© 2023 The Author(s).