微小RNA（miRNA）是一种小型非编码RNA，通过结合目标基因的3'非翻译区（UTR）在转录后调控基因表达。miRNA的异常表达可能导致多种疾病，包括癌症。结直肠癌（CRC）是全球癌症相关死亡的主要原因之一。miRNA的差异表达等多个因素可能对疾病进展有严重影响。本研究旨在计算地鉴定和实验验证可能影响CRC进展的强miRNA候选者。对公开可得的基因表达微阵列数据集进行的in silico分析显示CRC中miR-1183明显上调。将mRNA微阵列表达数据与预测的miR-1183靶基因进行比较，确定了细胞周期进展基因1（CCPG1）为强相关的miR-1183靶基因。定量聚合酶链反应（qPCR）的表达分析验证了在结直肠癌组织中miR-1183与CCPG1之间的逆相关关系。CCPG1通过与Dbs（特异性Rho鸟苷酸交换因子）的PH/DH结构域相互作用，间接调节细胞周期。有趣的是，计算分析还显示miR-1183在肝癌和胃癌中也上调。这一发现值得关注，因为肝脏和胃部是结直肠癌和肝细胞癌的主要转移部位。这一新发现凸显了miR-1183失调在原发性CRC之外的更广泛影响，可能作为有价值的预后标记和原发性和转移性CRC的治疗靶点。版权所有：© 2023 Fatima等。本文是根据创作共用许可证分发的开放获取文章，在保留原作者和出处的情况下，允许在任何媒介中自由使用、分发和复制。

MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression by binding to the 3' untranslated regions (UTR) of target genes. Aberrant expression of miRNAs can lead to disease, including cancer. Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths worldwide. Among several factors, differential expression of miRNA can have serious consequences on disease progression. This study was designed to computationally identify and experimentally verify strong miRNA candidates that could influence CRC progression. In silico analysis of publicly available gene expression microarray datasets revealed significant upregulation of miR-1183 in CRC. Comparison of mRNA microarray expression data with predicted miR-1183 targets led to the identification of cell cycle progression gene 1 (CCPG1) as strong, negatively correlated miR-1183 target. Expression analysis by means of quantitative PCR validated the inverse correlation between miR-1183 and CCPG1 in colorectal cancer tissues. CCPG1 indirectly modulates the cell cycle by interacting with the PH/DH domain of Dbs (Rho-specific guanine nucleotide exchange factor). Interestingly, the computational analysis also showed that miR-1183 is upregulated in liver and gastric cancer. This finding is notable as the liver and stomach are the primary metastatic sites for colorectal cancer and hepatocellular carcinoma respectively. This novel finding highlights the broader implications of miR-1183 dysregulation beyond primary CRC, potentially serving as a valuable prognostic marker and a therapeutic target for both primary and metastatic CRC.Copyright: © 2023 Fatima et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.