非酒精性脂肪肝病（NAFLD）是一个总称，指的是与脂肪沉积和肝脏组织损害相关的一组疾病。早期检测脂肪积累对于防止NAFLD进展为严重的病理阶段，如肝硬化和肝细胞癌至关重要。方法：我们利用传输-反射光声超声（TROPUS）的独特能力，结合了光学和声学对比的优势，对小鼠NAFLD的早期多参数评估进行了研究。结果：多光谱光声成像技术可以实现对肝组织中脂质含量的光谱分化，以及含氧和缺氧血红蛋白在体内的生物分布。脉冲回声（反射）超声成像进一步提供了有价值的解剖参考，而传输超声则便于在富含脂质区域中测绘声速变化，这与使用离体组织学染色检查的NAFLD肝脏中微囊性脂肪变性存在一致性。结论：所提出的多模态方法利用了多种光学和声学对比，有助于早期定量分析肝脏异常，为将TROPUS方法用于NAFLD的诊断和监测奠定了基础。©作者。

Non-alcoholic fatty liver disease (NAFLD) is an umbrella term referring to a group of conditions associated to fat deposition and damage of liver tissue. Early detection of fat accumulation is essential to avoid progression of NAFLD to serious pathological stages such as liver cirrhosis and hepatocellular carcinoma. Methods: We exploited the unique capabilities of transmission-reflection optoacoustic ultrasound (TROPUS), which combines the advantages of optical and acoustic contrasts, for an early-stage multi-parametric assessment of NAFLD in mice. Results: The multispectral optoacoustic imaging allowed for spectroscopic differentiation of lipid content, as well as the bio-distributions of oxygenated and deoxygenated hemoglobin in liver tissues in vivo. The pulse-echo (reflection) ultrasound (US) imaging further provided a valuable anatomical reference whilst transmission US facilitated the mapping of speed of sound changes in lipid-rich regions, which was consistent with the presence of macrovesicular hepatic steatosis in the NAFLD livers examined with ex vivo histological staining. Conclusion: The proposed multimodal approach facilitates quantification of liver abnormalities at early stages using a variety of optical and acoustic contrasts, laying the ground for translating the TROPUS approach toward diagnosis and monitoring NAFLD in patients.© The author(s).