乳腺癌是女性死亡的主要原因之一。钩藤是一种印度尼西亚的草药植物，可以作为一种抗癌药物。然而，草药的生物利用度较低，这影响了其生物活性。纳米包裹技术可以增加草药中生物活性化合物的生物利用度和稳定性。这项最新的研究试图揭示通过纳米包裹Na-藻酸盐的钩藤的抗癌和化学预防作用。使用紫外-可见光谱仪、傅里叶变换红外光谱、核磁共振和高分辨质谱技术，分离并表征了钩藤的生物活性化合物。使用Na-藻酸盐对钩藤提取物进行了纳米包裹。MTT法评估了钩藤纳米粒对T47D细胞的抗癌作用。同时，在苯并(α)吡咯诱导的乳腺癌小鼠中进行了化学预防分析。健康小鼠分为8组，包括对照组和治疗组。解析了钩藤乙酸乙酯提取物的高儿茶素含量，为89.34% (w/w)。成功表明了钩藤的纳米包裹(G-NPs)。G-NPs的粒径为78.40 ± 12.25 nm。G-NPs的装载效率(LE)和装载量(LA)分别为97.56 ± 0.04%和32.52 ± 0.01%。G-NPs的EC50值为10.39 ± 3.50 µg/mL，比对T47D细胞系的提取物的EC50值更有毒。给予苯并(α)吡咯诱导的小鼠200 mg/kg BW的G-NPs后，SOD和GSH水平分别为13.69 ng/mL和455.6 ng/mL。此外，最低的TNF-α水平为27.96 ng/mL。给予100 mg/kg BW的G-NPs可以最佳地增加7.18 ng/mL的CAT水平。在给予200 mg/kg BW的G-NPs小鼠的乳腺组织组织学分析中没有发现损伤或组织学异常。 © 2023 Wardana 等人。

Breast cancer is one of the main causes of death in women. Uncaria gambir is an Indonesian herbal plant that can be used as an anti-cancer. However, herbal medicines have low bioavailability, which affects their bioactivity. Nanoencapsulation can increase bioavailability and stability of bioactive compounds in herbal medicines.This recent finding tried to unravel anti-cancer and chemopreventive of U. gambir nano-encapsulated by Na-alginate.U. gambir bioactive compounds were isolated and characterized using UV-Vis spectrometer, FTIR, NMR and HR-MS. U. gambir extract was nanoencapsulated using Na-alginate. Anti-cancer effect was assessed by MTT assay towards T47D cell. Meanwhile, a chemopreventive analysis was carried out in breast cancer mice-induced benzo[α]pyrene. The healthy mice were divided into 8 groups comprising control and treatment.Elucidation of U. gambir ethyl acetate extract confirmed high catechin content, 89.34% (w/w). Successful nanoencapsulation of U. gambir (G-NPs) was indicated. The particle size of G-NPs was 78.40 ± 12.25 nm. Loading efficiency (LE) and loading amount (LA) of G-NPs were 97.56 ± 0.04% and 32.52 ± 0.01%, respectively. G-NPs had an EC50 value of 10.39 ± 3.50 µg/mL, which was more toxic than the EC50 value of extract towards the T47D cell line. Administration of 200 mg/kg BW G-NPs to mice induced by benzo[α]pyrene exhibited SOD and GSH levels of 13.69 ng/mL and 455.6 ng/mL. In addition, the lowest TNF-α level was 27.96 ng/mL. A dose of 100 mg/kg BW G-NPs could best increase CAT levels by 7.18 ng/mL. There was no damage or histological abnormalities found in histological analysis of the breast tissue in the group given 200 mg/kg BW G-NPs.© 2023 Wardana et al.