脂质体（LPs）是一种用于稳定具有有限使用的药物传递系统，因其会聚和融合的倾向而受到限制。解决这些问题的一种提议的方法是聚合物包覆。本研究调查了十八胺酸（ODA）包被脂质体和羧甲基壳聚糖（CMCS/ODA-LPs）用于提高油茶五环萜皂苷（WPTS）的转运能力的潜力。CMCS/ODA-LPs通过静电吸附和薄膜水合法制备。响应面方法（RSM）被应用于提高过程和最佳药物封装效率（Encapsulation Efficiency, EE）。合成的WPTS-CMCS/ODA-LPs在圆形形状中均匀分散，并在4℃存放14天期间，其粒径和形态没有显著变化。囊泡尺寸、zeta电位、多分散性指数（Polydispersity Index, PDI）和封装效率（%）分别为179.1±7.31 nm，-29.6±1.35mV，0.188±0.052和75.62±0.43。溶血试验表明WPTS-CMCS/ODA-LPs具有足够的生物相容性。与WPTS-LPs相比，WPTS-CMCS/ODA-LPs对癌细胞显示了更显著的细胞毒性作用。早期和WPTS-CMCS/ODA-LPs诱导的细胞凋亡导致的细胞死亡几乎是对照组的七倍。与生理pH 7.3相比，pH敏感的CMCS耦合脂质体在酸性pH 6.5条件下增加了药物释放。这些发现表明pH敏感的CMCS/ODA-LPs作为WPTS的治疗传递方法的有效性。

Liposomes (LPs) are a delivery system for stabilizing pharmaceuticals with limited use due to their propensity to congregate and fuse. A proposed method of addressing these is problems polymer coating. In this study, the potential of octadecylamine (ODA)-coated liposomes and carboxymethyl chitosan (CMCS/ODA-LPs) for enhancing wacao pentacyclic triterpene saponin (WPTS) transport capacity was investigated. CMCS/ODA-LPs were produced by electrostatic adsorption and thin-film hydration. Response surface methodology (RSM) was employed to enhance the process and encapsulation efficiency (EE) for optimum drug encapsulation efficiency. The synthesized WPTS-CMCS/ODA-LPs were uniformly dispersed in a circular shape, and during 14 days of storage at 4 °C, the particle size and morphology did not significantly change. Vesicle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (%) were 179.1 ± 7.31nm, -29.6 ± 1.35mV, 0.188 ± 0.052, and 75.62 ± 0.43, respectively. The hemolysis test revealed that WPTS-CMCS/ODA-LPs were sufficiently biocompatible. Compared to WPTS-LPs, WPTS-CMCS/ODA-LPs consistently showed a much more significant cytotoxic effect on cancer cells. Early and WPTS-CMCS/ODA-LPs-induced apoptosis resulted in almost seven times more cell death than the control. Compared to physiological pH 7.3, the pH-sensitive CMCS coupled liposomes increased drug release at acidic pH 6.5. These findings suggest the efficacy of pH-sensitive CMCS/ODA-LPs as a medication delivery method for WPTS.