背景：许多研究强调了G蛋白偶联受体（GPCRs）在肿瘤微环境（TME）重塑中的关键作用以及它们与肿瘤进展的相关性。然而，GPCRs与胶质母细胞瘤（GBM）中TME的关联尚未得到广泛探索。方法：在本研究中，我们利用单细胞RNA测序和批次测序的综合数据，调查了GBM中GPCRs的表达谱。从脑膜瘤和GBM患者中获得的手术样本进行了单细胞RNA测序，以检查GPCR水平和细胞相互作用。肿瘤微环境（TME）评分通过利用CIBERSORT进行浸润的免疫细胞计算得出。结果：我们的研究结果揭示了GBM中GPCRs的主要增加表达，并证明了GPCRs和TME的分类是GBM是独立的风险因素。肿瘤组织中GPCR表达高且TME评分低的患者显示了最差的预后，提示了潜在的侵袭性肿瘤表型。另一方面，肿瘤组织中GPCR表达低且TME评分高的患者显示了显着更好的预后，暗示了潜在更有利的肿瘤微环境。此外，该研究发现高GPCR水平的T细胞在单细胞RNA分析中与其他肿瘤和免疫细胞显示了广泛的细胞相互作用，表明它们可能参与了免疫逃逸。结论：综上所述，联合GPCRs和TME分类可作为GBM的预后标志物。GPCRs在GBM的肿瘤进展和TME中发挥了重要作用。机器翻译结束，如需修改请参考上文原文。Copyright © 2023 Guo, Yu, Gao, Ren, Zhang and Zheng.

Background: Numerous studies have highlighted the crucial role of G protein-coupled receptors (GPCRs) in tumor microenvironment (TME) remodeling and their correlation with tumor progression. However, the association between GPCRs and the TME in glioblastoma (GBM) remains largely unexplored. Methods: In this study, we investigated the expression profile of GPCRs in GBM using integrated data from single-cell RNA sequencing and bulk sequencing. Surgical samples obtained from meningioma and GBM patients underwent single-cell RNA sequencing to examine GPCR levels and cell-cell interactions. Tumor microenvironment (TME) score is calculated by the infiltrated immune cells with CIBERSORT. Results: Our findings revealed a predominantly increased expression of GPCRs in GBM, and demonstrated that the classification of GPCRs and TME is an independent risk factor in GBM. Patients with high GPCR expression in the tumor tissue and low TME score exhibited the worst outcomes, suggesting a potentially aggressive tumor phenotype. On the other hand, patients with low GPCR expression in the tumor tissue and high TME score showed significantly better outcomes, indicating a potentially more favorable tumor microenvironment. Furthermore, the study found that T cells with high GPCR levels displayed extensive cell-cell connections with other tumor and immune cells in the single cell RNA analysis, indicating their potential involvement in immune escape. Conclusion: In conclusion, GPCRs in combination with TME classification can serve as prognostic markers for GBM. GPCRs play an essential role in tumor progression and the TME in GBM.Copyright © 2023 Guo, Yu, Gao, Ren, Zhang and Zheng.