本研究旨在开发一种密集层状支架，作为一种仿生材料，具有在手术切除肿瘤后再生气管组织的潜在应用。支架是通过塑料压缩技术制备的，利用番石榴总酚化合物(PSID)作为潜在交联剂，在基于胶原(COL)、丝素蛋白(SF)和聚乙二醇400(PEG 400)的聚合物混合物中进行交联。傅里叶变换红外光谱(FTIR)和差示扫描量热法(DSC)证实了聚合物之间的化学相互作用以及TPC在COL和SF之间的交联。形态学分析显示支架具有多孔性、互连性和多孔表面结构，呈现出类似gyroid的几何形态。各向异性度分析结果表明，存在各向异性结构(0.1% TFC和0.3% TFC)和各向同性结构(0.5% TFC)。在力学性能方面，0.3% TFC配方的抗拉强度更高。TPC的添加百分比未导致显著差异(p > 0.05)在吸水能力和溶解速率方面。结果证实了TPC能够调控支架的形态学、形态尺度和力学性能。因此，本研究描述了一种潜在的新材料，用于改善手术切除肿瘤后主要气管结构的再生。© 2023. 作者。

The aim of this work was to develop a dense lamellar scaffold, as a biomimetic material with potential applications in the regeneration of tracheal tissue after surgical tumor resection. The scaffolds were produced by plastic compression technique, exploiting the use of total phenolic compounds (TPC) from Psidium guajava Linn as a potential cross-linking agent in a polymeric mixture based on collagen (COL), silk fibroin (SF), and polyethylene glycol 400 (PEG 400). Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) confirmed the chemical interactions between the polymers and the cross-linking of TPC between COL and SF. Morphological analyses showed scaffolds with porosity, interconnectivity, and a porous surface structure with a gyroid-like geometry. The analysis of the anisotropic degree resulted in anisotropic structures (0.1% TFC and 0.3% TFC) and an isotropic structure (0.5% TFC). In the mechanical properties, it was evidenced greater resistance for the 0.3% TFC formulation. The addition of TPC percentages did not result in a significant difference (p > 0.05) in swelling capacity and disintegration rate. The results confirmed that TPC were able to modulate the morphological, morphometric, and mechanical properties of scaffolds. Thus, this study describes a potential new material to improve the regeneration of major tracheal structures after surgical tumor removal.© 2023. The Author(s).