根据本研究的结果，已开发出一种新的电化学传感器，用于检测人类乳腺癌细胞（MCF-7）。以叶酸（FA）功能化的三嗪接枝还原氧化石墨烯（RGOTrz）作为玻璃碳电极（GCE）的修饰剂，作为感应元件。利用XRD（X射线衍射）、FT-IR（傅里叶变换红外光谱）、SEM（扫描电子显微镜）和UV-vis光谱研究了所得FA-RGOTrz / GCE 的成分。CV（循环伏安法）和EIS（电化学阻抗谱）技术也用于表征新电极的电化学性能。进一步，在磷酸缓冲溶液和[Fe（CN）6] 3-/ 4-作为合适的支持电解质和有用的探针的溶液中检查了MCF-7癌细胞。FA-RGOTrz / GCE提供了一个适当的基底来进行可逆性氧化还原反应，并且在与癌细胞结合后能提供良好的电化学信号。DPV（差分脉冲伏安法）结果表明，在[Fe（CN）6] 3-/ 4-存在下，叶酸受体（FR）在MCF-7细胞中与RGOTrz修饰电极结合，会还原叶酸，减弱电子转移并降低电流信号。测得的流速下，检测限为每毫升50个人类乳腺癌细胞。该FA-RGOTrz / GCE传感器凭借其独特的结构设计，显著提高了与MCF-7细胞的检测相关的电子转移和电化学活性。由于其特殊的结构，该FA-RGOTrz / GCE传感器对MCF-7细胞中的FR表现出很高的灵敏度，具有出色、可靠和令人满意的性能，在工业和医学领域有很大的应用前景。

Based on the results of this research, a new electrochemical sensor has been developed to detect human breast cancer cells (MCF-7). A folic acid (FA)-functionalized triazine-grafted reduced graphene oxide (RGOTrz) was used as the modifier of a glassy carbon electrode (GCE) for application as the sensing element. The composition of the resulting FA-RGOTrz/GCE was investigated using XRD (X-ray diffraction), FT-IR (Fourier-transform infrared) spectroscopy, SEM (scanning electron microscopy) and UV-vis spectroscopy studies. CV (cyclic voltammetry) and EIS (electrochemical impedance spectroscopy) techniques were also used to characterize the electrochemical proficiency of the new electrode. Further, MCF-7 cancer cells were examined in solutions of phosphate buffer and [Fe(CN)6]3-/4- as a suitable supporting electrolyte and a useful probe, respectively. The FA-RGOTrz/GCE provides a suitable substrate to reversible redox reactions and provides good electrochemical signals after binding to cancer cells. DPV (differential pulse voltammetry) results indicated that the binding of folate receptor (FR) in the MCF-7 cell to the RGOTrz-modified electrode, in the presence of [Fe(CN)6]3-/4-, reduced folic acid, diminished electron transfer and collapsed the current signal. During the measured flow, a detection limit of 50 human breast cancer cells per milliliter was obtained. The FA-RGOTrz/GCE, with its unique structural design, significantly increases the electron transfer and electrochemical activity towards the detection of MCF-7 cells. This FA-RGOTrz/GCE sensor, due to its special structure, shows high sensitivity to FR in MCF-7 cells and excellent, reliable and satisfactory performance and a great promise for use in industries and medical field.