乳腺癌是全球女性第二常见的癌症。引起肿瘤复发的主要因素是乳腺癌干细胞（BCSCs）的存在，它们能够逃避化疗和放疗。本研究调查了在MCF7和MDA-MB-231乳腺癌细胞系的BCSCs中过度表达的分泌型磷脂酶A2二型（sPLA2-IIA）的作用。此外，sPLA2-IIA的过度表达导致BCSCs中的EGFR/JNK/c-JUN/c-FOS信号增强，而sPLA2-IIA的沉默显著减少了两个细胞系中BCSCs的百分比并降低了信号传导。重要的是，sPLA2-IIA的沉默促使BCSCs的分化。结果显示，PET成像显示了BCSCs的转移潜力降低。我们的研究揭示了sPLA2-IIA在调节BCSCs中的新角色，它在调控BCSCs的分化和转移潜力中起着重要作用。版权所有 © 2023 Elsevier Inc. 发布。

Breast cancer is the second most cancer worldwide in females. The primary factor responsible for tumor recurrence is the presence of breast cancer stem cells (BCSCs), which escape the chemo-radiotherapy. In this study, we have investigated the role of Secretory phospholipase-A2 Group 2A (sPLA2-IIA) that is overexpressed in BCSCs of MCF7 and MDA-MB-231 breast cancer cell lines. Further, overexpression of sPLA2-IIA revealed an increased EGFR/JNK/c-JUN/c-FOS signaling in BCSCs, while sPLA2-IIA knockdown significantly reduced the percentage of BCSCs and decreased signaling in both the cell lines. Importantly, sPLA2-IIA knockdown showed differentiation of BCSCs. Strikingly, PET imaging showed a decreased metastatic potential of BCSCs. Our study revealed a novel role of sPLA2-IIA in regulating BCSCs, which play a crucial role in regulating the differentiation and metastatic potential of BCSCs.Copyright © 2023. Published by Elsevier Inc.