本研究对二巯基乙酮嘧啶基壳聚糖二苯乙烯醛席夫碱的合成和表征进行了重要的探索。采用了多种分析技术，包括紫外可见光谱、傅里叶变换红外光谱、X射线衍射、热重分析、差示扫描量热法、扫描电子显微镜和元素分析等，全面检查了这些衍生物。对一系列细菌和真菌菌株进行了壳聚糖衍生物的抗菌活性评估，同时对MCF-7、L-132和VERO细胞系进行了细胞毒性实验。在抗菌试验中，壳聚糖衍生物对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌表现出显著的抗菌性能，并对白色念珠菌和烟曲霉具有强效的抗真菌性能。细胞毒性评估显示，二巯基乙酮嘧啶基壳聚糖席夫碱（CHDBA）对L-132细胞系的效果显著，超过多柔比星的疗效2.44倍。此外，CHDBA对L-132和MCF-7细胞系表现出明显的活性，其IC50值分别为55.29μg/mL和185.8μg/mL。值得注意的是，壳聚糖衍生物对正常细胞系没有显示出细胞毒性，表明它们具有非毒性和安全可用性。根据这些发现，可以看出CHDBA具有进一步开发成为潜在的乳腺癌和肺癌治疗选择的潜力。版权所有 © 2023 Elsevier B.V. 发表。

This study holds significant importance as it explores the synthesis and characterization of two chitosan dibenzylideneacetone Schiff bases. Various analytical techniques, such as UV-Visible spectroscopy, FTIR, XRD, TGA, DSC, SEM, and elemental analysis, were employed to thoroughly examine these derivatives. The antimicrobial activity of the chitosan derivatives was evaluated against a range of bacterial and fungal strains, while cytotoxicity tests were conducted on MCF-7, L-132, and VERO cell lines. In the antimicrobial tests, the chitosan derivatives exhibited remarkable antibacterial properties against S. aureus, E. coli, and Pseudomonas aeruginosa, as well as potent antifungal properties against Candida albicans and Aspergillus fumigatus. The cytotoxicity assessment revealed that the dibenzylideneacetone chitosan Schiff base (CHDBA) showed significant effectiveness against the L-132 cell line, surpassing the efficacy of doxorubicin by 2.44 times. Moreover, it exhibited substantial activity against the L-132 and MCF-7 cell lines, with IC50 values of 55.29 μg/mL and 185.8 μg/mL, respectively. Notably, none of the chitosan derivatives demonstrated cytotoxicity towards the normal cell line, indicating their non-toxic nature and safe usability. Based on these findings, it is evident that CHDBA holds promise for further development as a potential treatment option for breast cancer and lung cancer.Copyright © 2023. Published by Elsevier B.V.