前瞻性辅助抗癌治疗的开发包括建立基于生物兼容和可降解载体的药物传递系统。我们设计了基于低酯化果胶水凝胶的薄膜和纳米颗粒（NPs），采用离子凝胶法。我们研究了形态学、纳米力学性质、生物相容性和抗癌活性。水凝胶薄膜通过果胶浓度和酯化程度（DE）来调节粘弹性和表面纳米架构，表现出可调控的孔隙频率和直径。体外研究表明，U87MG人类胶质母细胞瘤细胞系的代谢活性和增殖显著减少，可能通过黏附机制受到影响。神经胶质瘤细胞在完全脱酯化果胶薄膜上形成类神经球状团聚体，并且在50％酯化果胶制备的薄膜上不产生神经节突起。对果胶纳米颗粒的大小分布和纳米力学性质进行了检查。证实纳米颗粒呈球形，平均直径在90-115nm范围内变化，并且具有负电荷电位从-8.30到-7.86mV，表明其稳定性。纳米颗粒对细胞和代谢抑制没有毒性作用，显示出良好的生物相容性。在低酯化果胶上制备的纳米结构生物材料可能对辅助抗癌治疗和药物传递系统的设计具有重要意义。

Prospective adjuvant anticancer therapy development includes the establishing of drug delivery systems based on biocompatible and biodegradable carriers. We have designed films and nanoparticles (NPs) based on low-esterified pectin hydrogel using the ionic gelation method. We investigated morphology, nanomechanical properties, biocompatibility and anticancer activity. Hydrogel films are characterized by tunable viscoelastic properties and surface nanoarchitectonics through pectin concentration and esterification degree (DE), expressed in variable pore frequency and diameter. An in vitro study showed a significant reduction in metabolic activity and the proliferation of the U87MG human glioblastoma cell line, probably affected via the adhesion mechanism. Glioma cells formed neurosphere-like conglomerates with a small number of neurites when cultured on fully de-esterified pectin films and they did not produce neurites on the films prepared on 50% esterified pectin. Pectin NPs were examined in terms of size distribution and nanomechanical properties. The NPs' shapes were proved spherical with a mean diameter varying in the range of 90-115 nm, and a negative zeta potential from -8.30 to -7.86 mV, which indicated their stability. The NPs did not demonstrate toxic effect on cells or metabolism inhibition, indicating good biocompatibility. Nanostructured biomaterials prepared on low-esterified pectins could be of interest for biomedical applications in adjuvant anticancer therapy and for designing drug delivery systems.