红背木姜（AS），与白桦姜属相连，是一种药用真菌，具有抗炎、抗氧化和抑制肿瘤的特性。本研究调查了AS暴露对扭曲过度表达的头颈鳞状细胞癌（HNSCC，OECM-1和FaDu-Twist）细胞的代谢重编程和抗转移/上皮间质转变（EMT）效应。使用MTT分析、Western blot、迁移/侵袭分析、免疫荧光、葡萄糖摄取分析、乳酸分析、氧气消耗速率（OCR）/细胞外酸化速率（ECAR）分析、液相色谱-电喷雾离子化串联质谱（LC-ESI-MS）和qRT-PCR实验技术评估了AS治疗HNSCC细胞的治疗潜力。本研究显示，AS在扭曲过度表达的HNSCC细胞中表现出抗EMT和抗转移效应以及代谢重编程。AS暴露抑制了扭曲和缺氧诱导因子-1α（HIF-1α）在OECM-1和FaDu-Twist细胞中的蛋白质和/或mRNA表达。AS显著抑制了EMT，通过增强E-cadherin的表达，同时抑制N-cadherin的表达。此外，AS还通过减少OECM-1细胞中HIF-1α调节的糖酵解基因，降低了葡萄糖摄取和乳酸积累。AS减少了OECM-1细胞中的ECAR，并增加了氧气消耗速率、基础呼吸、ATP产生、最大呼吸和备用呼吸容量，在缺氧（CoCl2）和氧气（CoCl2）下。AS治疗在OECM-1细胞中降低了糖酵解中间产物的水平，同时增加了TCA循环代谢物的水平。我们得出结论，AS治疗通过抑制Twist和HIF-1α在扭曲过度表达的HNSCC细胞中抑制了EMT/转移和Warburg效应。版权所有 © 2023。由Elsevier B.V.出版。

Antrodia salmonea (AS), linked to the genus Taiwanofungus, is a medicinal fungus, and exhibits anti-inflammatory, anti-oxidant, and tumor inhibiting properties.In this study, we investigated the metabolic reprogramming and anti-metastasis/epithelial-mesenchymal transition (EMT) effects of AS exposure in Twist-overexpressing head and neck squamous cell carcinoma (HNSCC, OECM-1 and FaDu-Twist) cells.MTT assay, Western blot, migration/invasion assay, immunofluorescence, glucose uptake assay, lactate assay, oxygen consumption rate (OCR)/Extracellular acidification rate (ECAR) assay, Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS), and qRT-PCR experimental techniques were used to evaluate the therapeutic potential of AS treatment in HNSCC cells.This study showed that AS exhibits anti-EMT and anti-metastatic effects as well as metabolic reprogramming in Twist-overexpressing HNSCC cells. AS exposure inhibited Twist and hypoxia-inducible factor-1α (HIF-1α) protein and/or mRNA expression in Twist-overexpressing OECM-1 and FaDu-Twist cells. AS markedly suppressed EMT by enhancing the expression of E-cadherin; while the N-cadherin was suppressed. Furthermore, glucose uptake and lactate accumulation, together with HIF-1α-regulated glycolysis genes were diminished by AS in OECM-1 cells. AS decreased the ECAR, and enhanced the OCR together with basal respiration, ATP production, maximal respiration, and spare respiratory capacity under normoxia and hypoxia (CoCl2) in OECM-1 cells. There was a marked reduction in the level of glycolytic intermediate's; while TCA cycle metabolites were increased by AS treatment in OECM-1 cells.We concluded that AS treatment suppresses EMT/metastasis and Warburg effects through Twist and HIF-1α inhibition in Twist-overexpressing HNSCC cells.Copyright © 2023. Published by Elsevier B.V.