急性早幼粒细胞白血病（APL）是一种具有特定融合基因靶点的急性髓系白血病（AML），该融合基因为PML/RARα融合基因（PML/RARα），形成于染色体15和17的易位。检测PML/RARα是诊断、治疗调整、疗效评估、预后分析和复发预测APL的最可靠参数。本研究构建了一种快速的无酶检测PML/RARα的新型生物传感器，利用DNA酶和碳点/钴氧羟基纳米片复合体（CDs/CoOOH）。在检测系统中，分离的DNA酶能够特异性地通过与PML/RARα结合来形成完整的DNA酶，从而剪断发夹探针（HP），然后生成触发子，这是第一个信号放大。然后，触发子能够与捕获探针（CP）结合，锚定在链霉亲和素（SA）修饰的微孔板上，以及荧光猝灭信号探针（SP@CDs/CoOOH）。最后，加入抗坏血酸（AA）以分解CoOOH并释放CDs的荧光，这是第二个信号放大。通过DNA酶和CDs/CoOOH的双重信号放大，可以快速敏感地检测PML/RARα，克服了传统荧光方法中蛋白酶的限制，显示出在白血病的诊断和治疗中具有潜在临床应用价值。Copyright © 2023 Elsevier B.V. All rights reserved.

Acute promyelocytic leukemia (APL) is an acute myeloid leukemia (AML) with a specific fusion gene target, PML/RARα fusion gene (PML/RARα), which is formed by the translocation of chromosomes 15 and 17. Detection of PML/RARα is the most reliable parameter for the diagnosis, treatment adjustment, efficacy evaluation, prognosis analysis and relapse prediction of APL. In this study, a novel biosensor was constructed for rapid enzyme-free detection of PML/RARα using DNAzyme and carbon dots/cobalt oxhydroxide nanosheet complexs (CDs/CoOOH). In the detection system, the separated DNAzymes could specifically recognize and bind together by the PML/RARα to form a complete DNAzyme for shearing hairpin probe (HP), then generated trigger, which was the first signal amplification. Then, trigger could hybridize with the capture probe (CP) anchored to streptavidin (SA) modified microplate as well as fluorescence quenching signal probe (SP@CDs/CoOOH). Finally, ascorbic acid (AA) was added to decompose CoOOH and the fluorescence of CDs was released, which was the second signal amplification. Through the dual signal amplification of DNAzyme and CDs/CoOOH, PML/RARα could be detected quickly and sensitively, which overcame the limitation of protein enzyme in traditional fluorescence methods, showing potential clinical application value in the diagnosis and treatment of leukemia.Copyright © 2023 Elsevier B.V. All rights reserved.